Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002 Lugo, Spain.
Vet Parasitol. 2010 Aug 27;172(1-2):132-4. doi: 10.1016/j.vetpar.2010.04.029. Epub 2010 May 5.
Faecal specimens from five diarrhoeic goat kids (Capra hircus) younger than 21 days were collected in a goat farm in Galicia (NW Spain) and examined for the presence of Cryptosporidium oocysts. Two Cryptosporidium-positive isolates were detected and selected for molecular examination. A banding pattern indicative of Cryptosporidium bovis was obtained after restriction analyses of PCR products from small-subunit rRNA genes. However, both positive isolates were identified as Cryptosporidium xiaoi by sequence analyses of SSU rRNA and actin genes. Our results demonstrate that domestic goats are a host for C. xiaoi and suggest that this parasite species may be involved in the aetiology of neonatal diarrhoeic outbreaks in goat farms. This is the first published description of C. xiaoi in goat kids in Spain.
从加利西亚（西班牙西北部）一个山羊养殖场的 5 只小于 21 天龄的腹泻山羊幼崽中采集粪便样本，并检查是否存在隐孢子虫卵囊。检测到 2 个隐孢子虫阳性分离株，并选择用于分子检查。从小亚单位 rRNA 基因的 PCR 产物的限制分析获得指示牛隐孢子虫的带型。然而，通过 SSU rRNA 和肌动蛋白基因的序列分析，两个阳性分离株均被鉴定为微小隐孢子虫。我们的结果表明，家养山羊是微小隐孢子虫的宿主，并表明该寄生虫物种可能参与山羊养殖场新生仔猪腹泻暴发的病因。这是在西班牙首次报道山羊幼崽中的微小隐孢子虫。
