伏马菌素 B1 对马精子的毒性作用：对活力、精子染色质结构稳定性、ROS 产生和运动性的评估。

Toxic effects induced by mycotoxin fumonisin B1 on equine spermatozoa: assessment of viability, sperm chromatin structure stability, ROS production and motility.

机构信息

Institute of Sciences of Food Production (ISPA), National Council of Research (CNR), via Amendola 122/0, 70125 Bari, Italy.

出版信息

Toxicol In Vitro. 2010 Dec;24(8):2072-8. doi: 10.1016/j.tiv.2010.05.024. Epub 2010 Jun 9.

DOI:10.1016/j.tiv.2010.05.024

PMID:20541003

Abstract

Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium species that exerts its toxic effect through interference with the sphingolipid pathway by inhibiting ceramide synthase. A FB1-dependent sperm toxicity was reported in boars. No information on FB1-related reproductive toxicity in stallions, the most sensitive animal species, has been reported. The aim of the present study was to assess the in vitro toxicity of FB1 on fresh and frozen-thawed equine spermatozoa by analyzing sperm viability, chromatin stability (SCSA) and reactive oxygen species (ROS) production by flow cytometry and sperm motility by CASA system. Fumonisin B1 did not affect viability of fresh spermatozoa after 2h exposure up to 25 μM. Damage on sperm chromatin structure was observed only in one frozen sample after exposure up to 2.5 x 10⁻⁵ μM FB1 without associated increase of ROS. Increase of ROS, at FB1 levels up to 2.5 x 10⁻⁴ μM, was found on another frozen-thawed sperm sample, may be as a consequence of seminal plasma removal. At 7.5 and 15 μM, FB1 induced reduction of total and progressive motility.

摘要

伏马菌素 B1（FB1）是由镰刀菌属产生的一种真菌毒素，通过抑制神经酰胺合酶来干扰鞘脂代谢途径发挥其毒性作用。有报道称 FB1 可导致公猪精子毒性。然而，关于 FB1 对最敏感的动物种属——种马的生殖毒性，尚无相关信息。本研究旨在通过流式细胞术分析精子活力、染色质稳定性（SCSA）和活性氧（ROS）的产生以及 CASA 系统分析精子运动能力，评估 FB1 对新鲜和冷冻-解冻的马精子的体外毒性。在 2 小时的暴露时间内，FB1 浓度高达 25 μM 时，不会影响新鲜精子的活力。仅在一个冷冻样本中观察到暴露于 2.5 x 10⁻⁵ μM FB1 后精子染色质结构受损，而 ROS 没有增加。在另一个冷冻-解冻的精子样本中，ROS 增加，FB1 浓度高达 2.5 x 10⁻⁴ μM，可能是由于去除了精液中的血浆。在 7.5 和 15 μM 时，FB1 诱导总活力和前向运动活力降低。