鉴定铜绿假单胞菌抗代谢物 L-2-氨基-4-甲氧基-反式-3-丁烯酸的生物合成基因簇。
Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.
机构信息
Département de Microbiologie Fondamentale, Université de Lausanne, Lausanne, Switzerland.
出版信息
J Bacteriol. 2010 Aug;192(16):4251-5. doi: 10.1128/JB.00492-10. Epub 2010 Jun 11.
Abstract
L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction.
摘要
L-2-氨基-4-甲氧基-trans-3-丁烯酸（AMB）是铜绿假单胞菌产生的一种有效的抗生素和毒素。我们使用一种新的生化测定方法结合在 PAO1 菌株中的定点突变，鉴定了一个可能涉及硫模板机制的五个基因簇，该基因簇指定了 AMB 的生物合成。在天然不产 AMB 的菌株 PA7 中过表达这个基因簇导致了 AMB 的过量产生。
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