Zhong Bingwu, Wang Zhe, He Mingda
Department of Traditional Chinese Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2010 May;35(5):431-7. doi: 10.3969/j.issn.1672-7347.2010.05.005.
To observe the effect of Naoling decoction on hippocamal histomorphology and the expression of amyloid precursor protein (APP) in CA3 region in rats with Alzheimer's disease (AD), and to explore the therapeutical and the potential mechanism.
Forty SD rats were classified into 5 groups: a normal group, a sham-operated group, an AD group, a Naoling decoction group, and a Naofukang group. Alzheimer's disease model was established by Abeta1-42 injected into the hippocamal in the rats. The faculty of learning and memory was evaluated by Morris water maze. The changes of cell morphology were detected by HE staining. Expression of APP in CA3 region was mea-sured with immunohistochemical staining.
Morris water maze experiment showed that the escape latency of hidden platform in the AD group were delayed significantly (P<0.05) and the average times of passing was decreased (P<0.05). In the hippocampal CA3 field of the AD rats, HE staining and immunohistochemical test showed that pyramidal cells disturbed, neurons decreased significantly and expression of APP protein increased (P<0.05). Naoling decoction treatment improved pyramidal cellular disorders and decreased APP expression.
Naoling decoction can distinctly improve the learning and memory ability, and reduce the expression of APP in the AD model rats, suggesting that Naoling decoction can be used for the treatment of AD in rats.
观察脑灵汤对阿尔茨海默病（AD）大鼠海马组织形态学及CA3区淀粉样前体蛋白（APP）表达的影响，探讨其治疗作用及潜在机制。
将40只SD大鼠分为5组：正常组、假手术组、AD组、脑灵汤组和脑复康组。通过向大鼠海马注射β淀粉样蛋白1-42建立AD模型。采用Morris水迷宫评估学习记忆能力。通过HE染色检测细胞形态变化。用免疫组织化学染色法检测CA3区APP的表达。
Morris水迷宫实验显示，AD组隐藏平台逃避潜伏期显著延长（P<0.05），穿越次数减少（P<0.05）。AD大鼠海马CA3区HE染色及免疫组化检测显示，锥体细胞紊乱，神经元显著减少，APP蛋白表达增加（P<0.05）。脑灵汤治疗可改善锥体细胞紊乱，降低APP表达。
脑灵汤能明显提高AD模型大鼠的学习记忆能力，降低APP表达，提示脑灵汤可用于治疗大鼠AD。
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