[3H]ryanodine与兔脑微粒体结合的特性
Characteristics of [3H]ryanodine binding to the rabbit cerebral microsomes.
作者信息
Kawai T, Ishii Y, Imaizumi Y, Watanabe M
机构信息
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
出版信息
Brain Res. 1991 Feb 1;540(1-2):331-4. doi: 10.1016/0006-8993(91)90531-y.
Specific binding of [3H]ryanodine to the rabbit cerebral microsomes was dependent on free Ca2+ at micromolar concentrations and significantly increased by AMP-PNP and caffeine. Scatchard analysis showed a high and a low affinity binding site. The results suggest the presence of ryanodine binding sites which are activated by Ca2+ but with low efficacy, and greatly modified by adenine nucleotide, Mg2+ and also by caffeine.
[3H]ryanodine与兔脑微粒体的特异性结合依赖于微摩尔浓度的游离Ca2+，并被AMP-PNP和咖啡因显著增强。Scatchard分析显示存在一个高亲和力结合位点和一个低亲和力结合位点。结果表明存在ryanodine结合位点，其被Ca2+激活但效率较低，并被腺嘌呤核苷酸、Mg2+以及咖啡因显著改变。
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