Kurita Water Industries LTD., Gochoyama, Kawada, Nogi-machi, Shimotsuga-gun, Tochigi 3290105, Japan.
Water Res. 2010 Jul;44(14):4195-203. doi: 10.1016/j.watres.2010.05.021. Epub 2010 May 24.
A novel nitritation method based on the addition of inorganic carbon (IC) was verified using an airlift-fluidized bed reactor packed with sponge cubes. A continuous-treatment experiment demonstrated that the type of nitrification-nitrite or nitrate accumulation-could be controlled by the addition of different alkalinity sources (NaHCO(3) or NaOH, respectively). The maximum rate of ammonia oxidation at 30 degrees C was 2.47kg-N/(m(3) d), with nitrate formation of less than 0.5% of the converted ammonia. Nitrite accumulation of over 90% was maintained stably over 250 days at 30 degrees C and was achieved even at 19 degrees C. Qualitative and quantitative shifts of nitrifying bacteria in the biofilm were monitored by real-time PCR and T-RFLP analysis. Ammonia-oxidizing bacteria (AOB) were dominant but nitrite-oxidizing bacteria (NOB) were eliminated in the reactor when NaHCO(3) was used as the alkalinity source. From the kinetic data, we inferred that high IC concentrations drive stable nitritation by promoting a higher growth rate for AOB than for NOB.
采用海绵块填充的气升式流化床反应器验证了一种基于添加无机碳（IC）的新型亚硝化方法。连续处理实验表明，通过添加不同的碱度源（分别为 NaHCO(3)或 NaOH）可以控制硝化-亚硝酸盐积累或硝酸盐积累的类型。在 30°C 时，氨氧化的最大速率为 2.47kg-N/（m(3) d），转化的氨中硝酸盐的形成小于 0.5%。在 30°C 下稳定保持亚硝酸盐积累超过 250 天，甚至在 19°C 下也能实现。通过实时 PCR 和 T-RFLP 分析监测生物膜中硝化细菌的定性和定量变化。当使用 NaHCO(3)作为碱度源时，氨氧化菌（AOB）占优势，但在反应器中消除了亚硝酸盐氧化菌（NOB）。根据动力学数据，我们推断高 IC 浓度通过促进 AOB 的比 NOB 更高的增长率来驱动稳定的亚硝化。
