留尼汪岛拟暗果蝇自然种群中线粒体DNA异质性的维持。

Mitochondrial DNA heteroplasmy maintained in natural populations of Drosophila simulans in Réunion.

作者信息

Matsuura E T, Fukuda H, Chigusa S I

机构信息

Department of Biology, Ochanomizu University, Tokyo, Japan.

出版信息

Genet Res. 1991 Apr;57(2):123-6. doi: 10.1017/s0016672300029189.

DOI:10.1017/s0016672300029189

PMID:2055455

Abstract

Mitochondrial DNA (mtDNA) variation in Drosophila simulans was studied to determine whether the cytoplasmic state of mtDNA heteroplasmy persists in natural populations in Réunion. For this purpose, 172 isofemale lines, newly collected from two local populations, were examined, among which three types of mtDNA (siII, siIII and siIII') were found, based on the Hpa II restriction pattern. Ten of the lines were heteroplasmic for a combination of siII and siIII, as determined by autoradiography. The same type of heteroplasmy had been noted in one of the two local populations 8 years before (Satta et al. 1988). The present results suggest that the heteroplasmic state occurs recurrently in natural populations of D. simulans in Réunion.

摘要

对拟果蝇的线粒体DNA（mtDNA）变异进行了研究，以确定mtDNA异质性的细胞质状态在留尼汪岛的自然种群中是否持续存在。为此，检查了从两个当地种群新收集的172个同雌系，基于Hpa II限制性酶切图谱，在这些同雌系中发现了三种类型的mtDNA（siII、siIII和siIII'）。通过放射自显影确定，其中10个品系对siII和siIII的组合呈异质性。8年前在两个当地种群之一中也注意到了相同类型的异质性（Satta等人，1988年）。目前的结果表明，异质状态在留尼汪岛拟果蝇的自然种群中反复出现。