Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan.
Transgenic Res. 2011 Apr;20(2):261-70. doi: 10.1007/s11248-010-9412-7. Epub 2010 Jun 18.
Tomato leaf curl Taiwan virus (ToLCTWV) and Tomato spotted wilt virus (TSWV) are two major tomato viruses that cause serious economic losses. In this study, a partial C2 gene from ToLCTWV and the middle half of the N gene of TSWV were fused as a chimeric transgene to develop multiple virus resistance in transgenic plants. This construct was introduced into Nicotiana benthamiana and tomato by Agrobacterium-mediated transformation. Several transgenic lines showed no symptom post agro-inoculation with ToLCTWV and displayed high resistance to TSWV. The detection of siRNAs indicated that the resistance was via RNA silencing. This study demonstrated that linkage of gene segments from two viruses with distinct genomic organization, one DNA and the other RNA, can confer multiple virus resistance in transgenic plants via gene silencing.
番茄曲叶台湾病毒（ToLCTWV）和番茄斑萎病毒（TSWV）是两种导致严重经济损失的主要番茄病毒。在这项研究中，ToLCTWV 的部分 C2 基因和 TSWV 的 N 基因的中半部分融合为嵌合转基因，以在转基因植物中开发多种病毒抗性。该构建体通过农杆菌介导的转化被引入到黄花烟和番茄中。几个转基因系在 agro 接种后没有出现 ToLCTWV 的症状，并且对 TSWV 表现出高抗性。siRNAs 的检测表明这种抗性是通过 RNA 沉默实现的。本研究表明，来自两种具有不同基因组结构的病毒的基因片段的连接，一种是 DNA，另一种是 RNA，可以通过基因沉默在转基因植物中赋予多种病毒抗性。
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