Lu Yanxia, Liu Meng, Shi Shousen, Jiang Hong, Yang Lejin, Liu Xin, Zhang Qian, Pan Fang
Institute of Medical Psychology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.
J Asthma. 2010 Jun;47(5):526-31. doi: 10.3109/02770901003801964.
Although studies have shown that psychological stress has detrimental effects on bronchial asthma, there are few objective data on whether early-life stress, as early postnatal psychosocial environment, has a long-lasting effect on adult asthma and the potential pathophysiologic mechanism. This study aims to examine the effects on immune function and hypothalamic-pituitary-adrenal (HPA) axis responses in adult asthmatic rats that experienced stress in early life and the potential ameliorative effects of music therapy on these parameters.
Forty male Wistar rat pups were randomly assigned to the asthma group, the adulthood-stressed asthma group, the childhood-stressed asthma group, the music group, and the control group. Restraint stress and Mozart's Sonata K.448 were applied to ovalbumin (OVA)-induced asthmatic rats to establish psychological stress and music therapy models. The levels of serum corticosterone were examined in both childhood after stress and adulthood after OVA challenge. Immune indicators in blood, lung, and brain tissues were measured after the last OVA challenge.
Stress in both childhood and adulthood resulted in increases in leukocyte and eosinophil numbers and serum interleukin (IL)-4 levels. The adulthood-stressed group demonstrated increased corticosterone levels after challenge, whereas the childhood-stressed group showed increased corticosterone concentration in childhood but decreased level in adulthood. Central IL-1beta exhibited a similar tendency. Music group rats showed reduced serum IL-4 and corticosterone.
Stress in childhood and adulthood resulted in different HPA axis responsiveness in the exacerbation of markers of asthma. These data provide the first evidence of the long-term normalizing effects of music on asthmatic rats.
尽管研究表明心理压力对支气管哮喘有不利影响，但关于早期生活压力（如出生后早期的社会心理环境）是否对成人哮喘有长期影响以及潜在的病理生理机制，客观数据较少。本研究旨在探讨早期生活经历压力的成年哮喘大鼠的免疫功能和下丘脑 - 垂体 - 肾上腺（HPA）轴反应受到的影响，以及音乐疗法对这些参数的潜在改善作用。
将40只雄性Wistar幼鼠随机分为哮喘组、成年期应激哮喘组、儿童期应激哮喘组、音乐组和对照组。对卵清蛋白（OVA）诱导的哮喘大鼠施加束缚应激和莫扎特《第448号奏鸣曲》，以建立心理应激和音乐疗法模型。在应激后的儿童期以及OVA激发后的成年期检测血清皮质酮水平。在最后一次OVA激发后测量血液、肺和脑组织中的免疫指标。
儿童期和成年期的应激均导致白细胞和嗜酸性粒细胞数量以及血清白细胞介素（IL）-4水平升高。成年期应激组在激发后皮质酮水平升高，而儿童期应激组在儿童期皮质酮浓度升高，但成年期水平降低。中枢IL-1β表现出类似趋势。音乐组大鼠的血清IL-4和皮质酮降低。
儿童期和成年期的应激在哮喘标志物加重方面导致了不同的HPA轴反应性。这些数据首次证明了音乐对哮喘大鼠的长期正常化作用。
