Department of Neurology, Zhongnan Hospital, Wuhan University, No 169 Donghu Road, Wuchang District, Wuhan, China.
Curr Neurovasc Res. 2010 Aug;7(3):213-22. doi: 10.2174/156720210792231796.
Several nervous system injury models, such as sciatic crush and chronic cerebral hypoperfusion have been well studied in terms of neuroprotective effect of angelica injection. However, definitive experimental studies are lacking on diabetic peripheral neuropathy (DPN). This study sought to investigate the effects of angelica injection on DPN in type 1 diabetic rats. Diabetes was induced by single intraperitoneal injection of streptozotocin (STZ). To examine whether DPN model succeeded, tail-flick latency (TFL) and motor nerve conduction velocity (MNCV) were measured at 6 weeks after diabetes induction. Then, diabetic rats were treated with high- and low-dose angelica injection for 4 weeks. TFL, MNCV, morphology of sciatic nerve, myelinated nerve fiber density and the expressions of nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) in soleus and sciatic nerve were measured at 10 weeks after diabetes induction. The results showed the TFL was significantly shortened (p<0.001) and the MNCV was reduced (p<0.01) in diabetic rats compared with normal control rats at 6 weeks after diabetes induction. The TFL was obviously prolonged and the MNCV was further reduced in diabetic control group at 10 weeks after diabetes induction. TFL, MNCV and morphology of sciatic nerve were remarkably ameliorated and myelinated nerve fiber density and the expressions of NGF and BDNF in soleus and sciatic nerve were increased in the angelica treatment groups. This study suggests angelica injection has potential therapeutic effects on DPN, and the mechanism might be related to direct increase in NGF expression and direct or indirect increase in BDNF expression.
几种神经系统损伤模型，如坐骨神经挤压和慢性脑灌注不足，在当归注射液的神经保护作用方面已经得到了很好的研究。然而，对于糖尿病周围神经病变（DPN），还缺乏明确的实验研究。本研究旨在探讨当归注射液对 1 型糖尿病大鼠 DPN 的影响。糖尿病通过单次腹腔注射链脲佐菌素（STZ）诱导。为了检查 DPN 模型是否成功，在糖尿病诱导后 6 周测量尾巴拍打潜伏期（TFL）和运动神经传导速度（MNCV）。然后，用高剂量和低剂量当归注射液治疗糖尿病大鼠 4 周。在糖尿病诱导后 10 周测量 TFL、MNCV、坐骨神经形态、有髓神经纤维密度以及比目鱼肌和坐骨神经中神经生长因子（NGF）和脑源性神经营养因子（BDNF）的表达。结果显示，与正常对照组相比，糖尿病大鼠在糖尿病诱导后 6 周时 TFL 明显缩短（p<0.001），MNCV 降低（p<0.01）。糖尿病对照组在糖尿病诱导后 10 周时 TFL 明显延长，MNCV 进一步降低。当归治疗组 TFL、MNCV 和坐骨神经形态明显改善，比目鱼肌和坐骨神经中 NGF 和 BDNF 的有髓神经纤维密度和表达增加。本研究表明，当归注射液对 DPN 具有潜在的治疗作用，其机制可能与直接增加 NGF 表达以及直接或间接增加 BDNF 表达有关。
