2-羟丙基-β-环糊精以浓度依赖的方式从青蛙视杆感光细胞中去除全反式视黄醇。
2-Hydroxypropyl-beta-cyclodextrin removes all-trans retinol from frog rod photoreceptors in a concentration-dependent manner.
机构信息
Department of Physics, College of Charleston, Charleston, South Carolina 29425, USA.
出版信息
J Ocul Pharmacol Ther. 2010 Jun;26(3):245-8. doi: 10.1089/jop.2010.0020.
PURPOSE
To determine whether a nonprotein lipophilic carrier, 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD), can remove all-trans retinol from rod photoreceptor outer segments. All-trans retinol is generated in rod outer segments after light exposure. It is highly insoluble, and its efficient transport across extra- and intracellular aqueous space requires specialized carriers.
METHODS
Experiments were carried out with isolated frog rod photoreceptor cells. The removal of all-trans retinol by different concentrations of this carrier was measured by imaging its fluorescence in single-rod photoreceptors.
RESULTS
HP-beta-CD concentrations >0.3 mM significantly increased the rate of all-trans retinol removal. The rate of removal increased linearly with carrier concentration, with a slope of 0.0058 min(-1)/mM.
CONCLUSIONS
The effectiveness of HP-beta-CD shows that a specialized interaction with the cell membrane is not necessary for the efficient transfer of all-trans retinol between the cell membrane and the carrier. The transfer occurs through a collision-based mechanism, as indicated by the linear increase of the rate of removal with the carrier concentration.
目的
确定非蛋白亲脂载体 2-羟丙基-β-环糊精（HP-β-CD）是否能从视杆细胞外段中去除全反式视黄醇。视黄醇全反式是在光暴露后在视杆细胞外段中产生的。它的溶解度非常低，其在细胞内外水相中有效运输需要专门的载体。
方法
在分离的青蛙视杆感光细胞中进行实验。通过在单个视杆感光细胞中成像其荧光来测量不同浓度的这种载体对全反式视黄醇的去除率。
结果
HP-β-CD 浓度>0.3mM 可显著提高全反式视黄醇的去除率。去除率随载体浓度线性增加，斜率为 0.0058min(-1)/mM。
结论
HP-β-CD 的有效性表明，在细胞膜和载体之间有效转移全反式视黄醇不需要与细胞膜的特殊相互作用。这种转移是通过碰撞机制发生的，这表明去除率随载体浓度的线性增加。
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