Suppr超能文献

参与诱导植物系统抗性的铜绿假单胞菌 WCS358 的决定因素。

Determinants of Pseudomonas putida WCS358 involved in inducing systemic resistance in plants.

机构信息

Laboratory of Phytopathology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium.

出版信息

Mol Plant Pathol. 2005 Mar 1;6(2):177-85. doi: 10.1111/j.1364-3703.2005.00276.x.

Abstract

SUMMARY Pseudomonas putida WCS358 is a plant growth-promoting rhizobacterium originally isolated from the rhizosphere of potato. It can suppress soil-borne plant diseases by siderophore-mediated competition for iron, but it has also been reported to result in induced systemic resistance (ISR) in Arabidopsis thaliana. Bacterial determinants of this strain involved in inducing systemic resistance in Arabidopsis were investigated using a Tn5 transposon mutant defective in biosynthesis of the fluorescent siderophore pseudobactin, a non-motile Tn5 mutant lacking flagella, and a spontaneous phage-resistant mutant lacking the O-antigenic side chain of the lipopolysaccharides (LPS). When using Pseudomonas syringae pv. tomato as the challenging pathogen, purified pseudobactin, flagella and LPS all triggered ISR. However, the mutants were all as effective as the parental strain, suggesting redundancy in ISR-triggering traits in WCS358. The Botrytis cinerea-tomato, B. cinerea-bean and Colletotrichum lindemuthianum-bean model systems were used to test further the potential of P. putida WCS358 to induce ISR. Strain WCS358 significantly reduced disease development in all three systems, indicating that also on tomato and bean WCS358 can trigger ISR. In both tomato and bean, the LPS mutant had lost the ability to induce resistance, whereas the flagella mutant was still effective. In bean, the pseudobactin mutant was still effective, whereas this mutant has lost its effectivity in tomato. In both bean and tomato, flagella isolated from the parental strain were not effective, whereas LPS or pseudobactin did induce systemic resistance.

摘要

总结

恶臭假单胞菌 WCS358 是一种植物促生根际细菌,最初从马铃薯根际中分离得到。它可以通过铁载体介导的铁竞争来抑制土传植物病害,但也有报道称它会在拟南芥中引起诱导系统抗性(ISR)。使用 Tn5 转座子突变体、缺乏鞭毛的非运动 Tn5 突变体和自发噬菌体抗性突变体(缺乏脂多糖的 O-抗原侧链),研究了该菌株中参与诱导拟南芥系统抗性的细菌决定因素。当使用番茄丁香假单胞菌 pv. tomato 作为挑战病原体时,纯化的假杆菌素、鞭毛和 LPS 都能触发 ISR。然而,突变体与亲本菌株一样有效,这表明 WCS358 中触发 ISR 的性状存在冗余。使用灰葡萄孢番茄-番茄、灰葡萄孢-豆类和炭疽菌-豆类模型系统进一步测试了 P. putida WCS358 诱导 ISR 的潜力。菌株 WCS358 显著降低了所有三个系统中的疾病发展,表明 WCS358 也能在番茄和豆类上触发 ISR。在番茄和豆类中,LPS 突变体失去了诱导抗性的能力,而鞭毛突变体仍然有效。在豆类中,假杆菌素突变体仍然有效,而在番茄中,该突变体已经失去了其有效性。在豆类和番茄中,从亲本菌株分离的鞭毛无效,而 LPS 或假杆菌素能诱导系统抗性。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验