SCYNEXIS, P.O. Box 12878, Research Triangle Park, NC 27709, USA.
J Econ Entomol. 2010 Jun;103(3):726-34. doi: 10.1603/ec09254.
The southern chinch bug, Blissus insularis Barber (Hemiptera: Blissidae), is the most destructive insect pest of St. Augustinegrass, Stenotaphrum secundatum (Walt.) Kuntze. Management of B. insularis has depended on frequent insecticide applications, which has resulted in populations becoming resistant to several insecticide classes. To facilitate developing a resistance management program for this pest, it is necessary to develop methods to rear insects of known age, generation, and pesticide exposure history. Synchronized rearing methods were developed after testing five different laboratory methods. The use of glass jars and a combined diet of fresh corn, Zea mays L., cob and St. Augustinegrass proved to be best for producing B. insularis of known age and generation. Body size was consistent over nine generations of rearing. Production of a high proportion of brachypterous B. insularis (the nondispersal adult form) also suggests that populations were not stressed during laboratory rearing. This work presents the first successful synchronized rearing method for B. insularis.
南方臀纹粉蚧(Blissus insularis Barber)(半翅目:臀纹粉蚧科)是圣奥古斯丁草(Stenotaphrum secundatum (Walt.) Kuntze)最具破坏性的虫害之一。该虫害的管理一直依赖于频繁的杀虫剂使用,这导致了其种群对几种杀虫剂类别的抗性。为了便于为这种虫害制定抗性管理计划,有必要开发出一种能够饲养具有已知年龄、代次和农药暴露史的昆虫的方法。在测试了五种不同的实验室方法后,开发出了同步饲养方法。使用玻璃罐和新鲜玉米(Zea mays L.)穗、玉米芯和圣奥古斯丁草的组合饲料被证明是产生具有已知年龄和代次的南方臀纹粉蚧的最佳方法。在九代的饲养过程中,其体型大小保持一致。大量产生短翅型南方臀纹粉蚧(非扩散型成虫)也表明,在实验室饲养过程中,种群未受到压力。这项工作提出了南方臀纹粉蚧的第一个成功的同步饲养方法。