Fujii Y, Yokochi T, Nakashima I, Asai J, Kato N
Department of Bacteriology, Nagoya University School of Medicine, Aichi, Japan.
Med Microbiol Immunol. 1991;180(1):1-14. doi: 10.1007/BF00191695.
Experimental autoimmune orchitis (EAO) was induced in SMA mice (H-2nondefined) by repeated injection at intervals of 30 days of syngeneic testis homogenate (TH) together with Klebsiella O3 lipopolysaccharide (KO3 LPS) as a potent adjuvant. EAO was not induced by repeated injection of TH alone or KO3 LPS alone. At 10 days after the secondary injection of TH + KO3 LPS, there was marked infiltration with neutrophils in the seminiferous tubules and in the interstitium of the testis accompanied by destruction of the architecture of the seminiferous tubules and hypospermatogenesis. At 20 days after the secondary injection, infiltration with neutrophils in these areas had been replaced mostly by mononuclear cells (lymphocytes, plasma cells, and macrophages). Histopathological changes of the testes became severer by further injections until the 10th injection. The EAO lesions in the terminal stage were characterized by complete destruction of the tubular architecture of the testis, fibrosis, and aspermatogenesis. Lesions in the terminal stage were not restored at all. Spermagglutinating antibody titers in the serum increased and delayed-type hypersensitivity against TH estimated by footpad swelling developed in mice injected repeatedly with TH + KO3 LPS. Using immunofluorescence, antibodies against acrosomal components and tail components of the spermatozoa were detected in serum of these mice.
通过每隔30天重复注射同基因睾丸匀浆(TH)与肺炎克雷伯菌O3脂多糖(KO3 LPS)作为强效佐剂,在SMA小鼠(H-2未定义)中诱导实验性自身免疫性睾丸炎(EAO)。单独重复注射TH或单独注射KO3 LPS均未诱导出EAO。在第二次注射TH + KO3 LPS后10天,生精小管和睾丸间质中有明显的中性粒细胞浸润,伴有生精小管结构破坏和精子发生减少。在第二次注射后20天,这些区域的中性粒细胞浸润大多已被单核细胞(淋巴细胞、浆细胞和巨噬细胞)取代。通过进一步注射直至第10次注射,睾丸的组织病理学变化变得更加严重。终末期的EAO病变特征为睾丸管状结构完全破坏、纤维化和无精子发生。终末期病变完全没有恢复。在反复注射TH + KO3 LPS的小鼠中,血清中的精子凝集抗体滴度升高,并且通过足垫肿胀评估的对TH的迟发型超敏反应出现。使用免疫荧光法,在这些小鼠的血清中检测到针对精子顶体成分和尾部成分的抗体。