Yan Dong-mei, Zhu Hui, An Jun-jing
Organization Western Pacific Region Regional Reference Polio Laboratory, State Key Laboratory for Molecular Virology & Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhongguo Yi Miao He Mian Yi. 2010 Apr;16(2):105-9.
Application of Real Time Fluorescent quantitative reverse transcription polymerase chani reaction (rRT-PCR)to poliovirus identification in Chinese Poliomyelitis Laboratory Network and evaluation of the assay.
According to Real-time RT-PCR recommended by WHO and developed by USA Centers for Disease Control and Prevention, 10 poliovirus isolates from laboratories of Chinese poliomyelitis network were tested for intratypic differentiation (ITD) and vaccine derived polioviruses (VDPVs) screening. The results of Real-time RT-PCR for 10 isolates were compared with those of VP, region sequencing.
The Real-time RT-PCR results for 10 isolates did not completely consist with those of VP1 region sequencing. 5 Pre-VDPVs can not be identified by Real-time RT-PCR and type IVDPV from Shanxi province in 2009 was missed by the assay.
The Real-time RT-PCR retrospective and prospective researches for large scale of polioviruses well be conducted to determine if the assay is applicable to Chinese Poliomyelitis Laboratory Network.
应用实时荧光定量逆转录聚合酶链反应（rRT-PCR）对中国脊髓灰质炎实验室网络中的脊髓灰质炎病毒进行鉴定并评估该检测方法。
根据世界卫生组织推荐并由美国疾病控制与预防中心研发的实时逆转录聚合酶链反应，对来自中国脊髓灰质炎网络实验室的10株脊髓灰质炎病毒分离株进行型内鉴别（ITD）和疫苗衍生脊髓灰质炎病毒（VDPV）筛查。将10株分离株的实时逆转录聚合酶链反应结果与VP1区测序结果进行比较。
10株分离株的实时逆转录聚合酶链反应结果与VP1区测序结果不完全一致。5株疫苗衍生脊髓灰质炎病毒前期病毒无法通过实时逆转录聚合酶链反应鉴定，该检测方法遗漏了2009年山西省的1株疫苗衍生脊髓灰质炎病毒。
将对大量脊髓灰质炎病毒进行实时逆转录聚合酶链反应的回顾性和前瞻性研究，以确定该检测方法是否适用于中国脊髓灰质炎实验室网络。
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