Department of Physiology, College of Oriental Medicine, Kyung Hee University #1 Hoeki-dong Dongdaemoon-gu, Seoul 130-701, Republic of Korea.
Cytokine. 2010 Sep;51(3):259-65. doi: 10.1016/j.cyto.2010.05.011. Epub 2010 Jun 26.
To evaluate the direct effects of Cyperi Rhizoma (CR), a plant water extract, on Th1/Th2 lineage development in vitro, this study was conducted. Sorted CD4(+) T cells obtained from the splenocytes of BALB/c mice were activated with anti-CD3/anti-CD28 and then cultured in medium that contained CR medium under Th1 inducing or Th2 inducing conditions. Subsequently, IFN-gamma or IL-4 secreting cells were quantitated using flow cytometry analysis. In addition, IFN-gamma and IL-4 protein secretions were detected by ELISA analysis, after which, IFN-gamma and T-bet transcripts, key players in the Th1 immune function, and also, IL-4 and GATA-3, which are primary components in the Th2 immune mechanism, were quantitated by real-time RT-PCR. CR had no mitogenic effects on un-stimulated CD4(+) T cells, however, it increased the CD4(+) T cell population. Th1/Th2 polarization experiments revealed that CR enhanced IFN-gamma secretion in Th1 cells, but reduced the IL-4 in Th2 cells, and this occurred in a dose-dependent manner and showed significances. In addition, under Th1/Th2 skewed conditions, the transcription levels of IFN-gamma and T-bet were considerably increased, while the expressions of IL-4 and GATA-3 were relatively decreased with CR treatment. These findings suggest that CR enhances Th1 lineage development by increasing Th1 specific cytokine expression and secretion and reduces Th2 lineage development by repressing Th2 specific cytokine productions. Therefore, CR extract may be useful for preventing the onset of allergies or improving allergic symptoms.
为了评估 Cyperi Rhizoma(CR)植物水提取物在体外对 Th1/Th2 谱系发育的直接影响,进行了这项研究。从 BALB/c 小鼠的脾细胞中分离出 CD4(+) T 细胞,用抗 CD3/抗 CD28 激活,然后在含有 CR 培养基的 Th1 诱导或 Th2 诱导条件下进行培养。随后,使用流式细胞术分析定量 IFN-γ或 IL-4 分泌细胞。此外,通过 ELISA 分析检测 IFN-γ和 IL-4 蛋白分泌,之后通过实时 RT-PCR 定量 IFN-γ和 T-bet 转录物,这些是 Th1 免疫功能的关键因子,以及 IL-4 和 GATA-3,它们是 Th2 免疫机制的主要成分。CR 对未刺激的 CD4(+) T 细胞没有有丝分裂作用,但增加了 CD4(+) T 细胞群体。Th1/Th2 极化实验表明,CR 增强了 Th1 细胞中 IFN-γ的分泌,但减少了 Th2 细胞中的 IL-4,这是剂量依赖性的,并且具有统计学意义。此外,在 Th1/Th2 倾斜条件下,CR 处理后 IFN-γ和 T-bet 的转录水平显著增加,而 IL-4 和 GATA-3 的表达相对减少。这些发现表明,CR 通过增加 Th1 特异性细胞因子的表达和分泌来增强 Th1 谱系的发育,通过抑制 Th2 特异性细胞因子的产生来减少 Th2 谱系的发育。因此,CR 提取物可能有助于预防过敏的发生或改善过敏症状。