蛋白质酪氨酸磷酸酶及其底物分析
Analysis of protein tyrosine phosphatases and substrates.
作者信息
Mercan Fatih, Bennett Anton M
机构信息
Yale University School of Medicine, New Haven, Connecticut, USA.
出版信息
Curr Protoc Mol Biol. 2010 Jul;Chapter 18:Unit 18.16. doi: 10.1002/0471142727.mb1816s91.
Abstract
Protein tyrosine phosphorylation is a reversible post-translational modification that is essential for life in eukaryotic cells. The combinatorial action of both protein tyrosine kinases and protein tyrosine phosphatases (PTPs) determines the net level of cellular tyrosine phosphorylation. This unit discusses methods to determine the level of protein tyrosine phosphatase activity and methods for discovering novel substrates for protein tyrosine phosphatases.
摘要
蛋白质酪氨酸磷酸化是一种可逆的翻译后修饰，对真核细胞的生命活动至关重要。蛋白质酪氨酸激酶和蛋白质酪氨酸磷酸酶（PTP）的联合作用决定了细胞酪氨酸磷酸化的净水平。本单元讨论了测定蛋白质酪氨酸磷酸酶活性水平的方法以及发现蛋白质酪氨酸磷酸酶新底物的方法。
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