抗磷酸丙糖异构酶抗体与 MRL/MpJ-Faslpr 小鼠的关联。

Association of anti-triosephosphate isomerase antibody and MRL/MpJ-Faslpr mouse.

机构信息

Fukushima Medical University, Department of Gastroenterology and Rheumatology, School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan.

出版信息

J Neuroimmunol. 2010 Sep 14;226(1-2):110-5. doi: 10.1016/j.jneuroim.2010.05.041. Epub 2010 Jun 26.

DOI:10.1016/j.jneuroim.2010.05.041

PMID:20584554

Abstract

The association between anti-triosephosphate isomerase (TPI) antibodies and MRL/MpJ-Fas(lpr) (MRL/lpr) mice was examined. We found that serum anti-TPI antibody levels in MRL/lpr mice, measured by enzyme-linked immunosorbent assay, were significantly higher than that of age-matched Balb/c mice and NZB/WF1 mice. Anti-TPI antibodies were detected in serum and cerebrospinal fluid in MRL/lpr mice by Western blotting. Inoculation of anti-TPI monoclonal antibody-producing hybridoma into the brain of Balb/c mice resulted in immunoglobulin deposition in the regions near the ventricles, hippocampus, and choroid plexus. Anti-TPI antibodies may play a role in the etiology of brain damage and behavioral deficits in MRL/lpr mice.

摘要

研究了抗磷酸三糖异构酶（TPI）抗体与 MRL/MpJ-Fas(lpr)（MRL/lpr）小鼠之间的关系。我们发现，通过酶联免疫吸附试验测量的 MRL/lpr 小鼠血清中抗 TPI 抗体水平明显高于同龄的 Balb/c 小鼠和 NZB/WF1 小鼠。通过 Western blot 在 MRL/lpr 小鼠的血清和脑脊液中检测到抗 TPI 抗体。将产生抗 TPI 单克隆抗体的杂交瘤注入 Balb/c 小鼠的大脑中，导致免疫球蛋白在脑室附近、海马体和脉络丛的区域沉积。抗 TPI 抗体可能在 MRL/lpr 小鼠的脑损伤和行为缺陷的发病机制中发挥作用。