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紫外线下共振光栅生物芯片的二维无标记成像。

2D label-free imaging of resonant grating biochips in ultraviolet.

作者信息

Bougot-Robin K, Reverchon J-L, Fromant M, Mugherli L, Plateau P, Benisty H

机构信息

Thales Research & Technology, 1 avenue Fresnel, Campus Polytechnique, 91767 Palaiseau, France.

出版信息

Opt Express. 2010 May 24;18(11):11472-82. doi: 10.1364/OE.18.011472.

Abstract

2D images of label-free biochips exploiting resonant waveguide grating (RWG) are presented. They indicate sensitivities on the order of 1 pg/mm2 for proteins in air, and hence 10 pg/mm2 in water can be safely expected. A 320x256 pixels Aluminum-Gallium-Nitride-based sensor array is used, with an intrinsic narrow spectral window centered at 280 nm. The additional role of characteristic biological layer absorption at this wavelength is calculated, and regimes revealing its impact are discussed. Experimentally, the resonance of a chip coated with protein is revealed and the sensitivity evaluated through angular spectroscopy and imaging. In addition to a sensitivity similar to surface plasmon resonance (SPR), the RWGs resonance can be flexibly tailored to gain spatial, biochemical, or spectral sensitivity.

摘要

本文展示了利用共振波导光栅(RWG)的无标记生物芯片的二维图像。这些图像表明,对于空气中的蛋白质,灵敏度约为1 pg/mm²,因此在水中可安全预期灵敏度为10 pg/mm²。使用了一个基于铝镓氮化物的320×256像素传感器阵列,其固有窄光谱窗口中心位于280 nm。计算了该波长下特征生物层吸收的额外作用,并讨论了揭示其影响的机制。通过实验揭示了涂有蛋白质的芯片的共振,并通过角谱和成像评估了灵敏度。除了具有与表面等离子体共振(SPR)相似的灵敏度外,RWG的共振还可以灵活调整,以获得空间、生化或光谱灵敏度。

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