Center for Vaccines and Immunity, Research Institute at Nationwide Children's Hospital, Columbus, Ohio 43205, USA.
J Virol. 2010 Sep;84(17):8975-9. doi: 10.1128/JVI.00571-10. Epub 2010 Jun 30.
It is still unknown whether a noninfectious gammaherpesvirus vaccine is able to prevent or reduce virus persistence. This led us to use dendritic cells loaded with tumor B cells as a vaccine approach for the murine gammaherpesvirus 68 (gammaHV68) model of infection. Dendritic cells loaded with UV-irradiated latently infected tumor B cells induce broad, strong, and long-lasting immunity against gammaHV68. Dendritic cell vaccination prevents the enlargement of lymph nodes and severely limits acute infection and early latency but does not prevent gammaHV68 from establishing long-term latency. Our findings support the concept that attenuated viruses may be the best vaccine option for preventing gammaherpesvirus persistence.
目前尚不清楚非传染性γ疱疹病毒疫苗是否能够预防或减少病毒持续感染。这促使我们使用负载肿瘤 B 细胞的树突状细胞作为感染小鼠γ疱疹病毒 68(γHV68)模型的疫苗接种方法。负载经紫外线照射潜伏感染肿瘤 B 细胞的树突状细胞可诱导针对γHV68 的广泛、强烈和持久的免疫。树突状细胞疫苗接种可防止淋巴结肿大,并严重限制急性感染和早期潜伏,但不能防止 γHV68 建立长期潜伏。我们的研究结果支持这样的概念,即减毒病毒可能是预防γ疱疹病毒持续感染的最佳疫苗选择。