Reproductive Medicine Center, Peking University Third Hospital, Beijing, 100083, China.
Sci China Life Sci. 2010 May;53(5):581-9. doi: 10.1007/s11427-010-0111-7. Epub 2010 May 23.
Several approaches have been used to encourage the differentiation of cardiomyocytes from human embryonic stem cells. However, the differentiation efficiency is low, and appropriate culture protocols are needed to produce adequate numbers of cardiomyocytes for therapeutic cell transplantation. This study investigated the effects of serum on cardiomyocyte differentiation in suspension culture medium during embryoid body (EB) formation by human embryonic stem cells. The addition of ascorbic acid, dimethylsulfoxide and 5-aza-2'-deoxycytidine during days 5-7 at the EB-forming stage resulted in an increase in the numbers of rhythmically contracting clusters of derived cardiomyocytes. Treatment with 0.1 mmol L(-1) ascorbic acid alone, or more notably in combination with 10 micromol L(-1) 5-aza-2'-deoxycytidine, induced the formation of beating cells within EBs. Most of the beating clusters had spontaneous contraction rates similar to those found in human adults, and their contractile activity lasted for up to 194 days.
已经采用了几种方法来促使人心肌细胞从人胚胎干细胞分化。然而，分化效率低，需要适当的培养方案来产生足够数量的可用于治疗性细胞移植的心肌细胞。本研究调查了在人胚胎干细胞形成胚状体（EB）过程中悬浮培养液中血清对心肌细胞分化的影响。在 EB 形成阶段的第 5-7 天添加抗坏血酸、二甲基亚砜和 5-氮杂-2'-脱氧胞苷，可增加节律性收缩的衍生心肌细胞簇的数量。单独用 0.1 mmol/L 抗坏血酸处理，或更显著的是与 10 微摩尔/L 5-氮杂-2'-脱氧胞苷联合处理，可诱导 EB 内形成搏动细胞。大多数搏动簇的自发收缩率与人成年相似，其收缩活性可持续长达 194 天。
