Two new genes (smhA and lytE) apparently functionally related to the murH gene of Escherichia coli.

The murH mutant of Escherichia coli exhibits temperature-sensitive growth and lysis at the restrictive temperature. Temperature-resistant derivatives of the mutant occurred at a frequency of about 3 X 10(-6). All of the seven independent isolates examined were shown to be pseudorevertants carrying extragenic suppressors of murH, which mapped at 24.5 min on the linkage map. One allele, apparently representing a new locus, designated smhA, was characterized further. The smhA mutation by itself conferred no recognizable phenotype. However, smhA suppressed the temperature-sensitive lysis phenotype of the murH mutant. The smhA mutant acquired a spontaneous mutation in another new gene, designated lytE, which was mapped at 25 min. The lytE mutation by itself conferred a temperature-sensitive lysis phenotype indistinguishable from that of the murH mutant. The lytE mutation was suppressed by smhA as well as by another suppressor of murH designated smhB. The suppressor activity of smhA was apparently relatively specific in that smhA failed to prevent lysis caused by either mutational or antibiotic-induced blocks in peptidoglycan synthesis. The possibility that the smhA and lytE genes are functionally related to murH is considered.