迁移刺激因子 (MSF) 通过抑制 AKT 促进成纤维细胞迁移。
Migration Stimulating Factor (MSF) promotes fibroblast migration by inhibiting AKT.
机构信息
Unit of Cell and Molecular Biology, The Dental School, College of Medicine, Dentistry and Nursing, University of Dundee, Park Place, Dundee DD1 4HR, UK.
出版信息
Cell Signal. 2010 Nov;22(11):1655-9. doi: 10.1016/j.cellsig.2010.06.005. Epub 2010 Jul 1.
The protein kinase AKT is activated strongly by many motogenic growth factors, yet has recently been shown capable of inhibiting migration in several cell types. Here we report that treatment with Migration Stimulating Factor (MSF), a truncated form of fibronectin that promotes the migration of many cell types, inhibits AKT activity in human fibroblasts and endothelial cells. In fibroblasts, treatment with either MSF or the AKT inhibitor, Akti-1/2, stimulated migration into 3D collagen gels to a similar extent and the effects of Akti-1/2 on migration could be blocked by the expression of an inhibitor-resistant mutant, AKT1 W80A. These data indicate that MSF promotes fibroblast migration, at least in part, by inhibiting the activity of AKT.
蛋白激酶 AKT 可被多种促迁移生长因子强烈激活，但最近的研究表明其能够抑制多种细胞类型的迁移。在这里，我们报告说，迁移刺激因子（MSF）处理（一种促多种细胞类型迁移的纤维连接蛋白的截断形式）抑制人成纤维细胞和内皮细胞中的 AKT 活性。在成纤维细胞中，MSF 或 AKT 抑制剂 Akti-1/2 的处理以相似的程度刺激向 3D 胶原凝胶中的迁移，并且 Akti-1/2 对迁移的影响可以通过表达抑制剂抗性突变体 AKT1 W80A 来阻断。这些数据表明，MSF 通过抑制 AKT 的活性促进成纤维细胞迁移，至少部分如此。
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