通过化学连接探测DNA三螺旋结构

Probing DNA triple helix structure by chemical ligation.

作者信息

Dolinnaya N G, Pyatrauskene O V, Shabarova Z A

机构信息

A.N. Belozersky Laboratory and Chemical Department, Moscow State University, USSR.

出版信息

FEBS Lett. 1991 Jun 24;284(2):232-4. doi: 10.1016/0014-5793(91)80692-v.

DOI:10.1016/0014-5793(91)80692-v

PMID:2060640

Abstract

A series of oligomeric double and triple helical DNAs with irregular sequences of homopurine and homopyrimidine strands were prepared. DNA triplexes were identified by CD spectroscopy and thermal denaturation profiles (biphasic helix-coil transition). Condensation of oligonucleotides on single and double-stranded DNA templates was performed using water-soluble carbodiimide, phosphodiester and pyrophosphate internucleotide bonds being newly formed. Such chemical ligation proved to be a sensitive monitor of changes in the sugar-phosphate backbone resulting from conversion of double to triple helix and of third-strand binding.

摘要

制备了一系列具有同型嘌呤链和同型嘧啶链不规则序列的寡聚双链和三链螺旋DNA。通过圆二色光谱和热变性曲线（双相螺旋-卷曲转变）鉴定DNA三链体。使用水溶性碳二亚胺在单链和双链DNA模板上进行寡核苷酸缩合，新形成磷酸二酯键和焦磷酸核苷酸间键。这种化学连接被证明是监测由双链向三链螺旋转变以及第三链结合引起的糖-磷酸主链变化的灵敏方法。