Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University, Busan, Korea.
Arch Pharm Res. 2010 Jun;33(6):959-66. doi: 10.1007/s12272-010-0620-8. Epub 2010 Jul 6.
Paeoniflorin (PF), a monoterpene glucoside, is a primary bioactive component of paeony, the root extract of Paeonia lactiflora. We tested the antioxidant effects of PF and its ability to prevent lipopolysaccharide (LPS)-induced oxidative stress. We intraperitoneally administered PF (2.5, 5, or 10 mg/kg) to rats for 20 days. On day 21, we injected the rats with LPS 4 h before sacrifice and measured serum levels of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, lactate dehydrogenase as well as the levels of malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase in liver whole-cell homogenates and mitochondrial fractions. LPS treatment increased levels of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, lactate dehydrogenase, and malondialdehyde, but PF treatment blocked these increases. LPS treatment also decreased antioxidant levels of superoxide dismutase, catalase, and glutathione peroxidase, but PF blocked these decreases. PF also protected liver tissue, as shown by histopathology. These results suggest that PF can protect against LPS-induced liver inflammation.
芍药苷（PF）是芍药的主要生物活性成分，是芍药根的提取物，属于单萜糖苷。我们检测了 PF 的抗氧化作用及其预防脂多糖（LPS）诱导的氧化应激的能力。我们给大鼠腹腔内注射 PF（2.5、5 或 10 mg/kg），连续 20 天。第 21 天，在处死前 4 小时给大鼠注射 LPS，并测量血清中谷氨酸草酰乙酸转氨酶、谷氨酸丙酮酸转氨酶、乳酸脱氢酶的水平，以及肝全细胞匀浆和线粒体部分中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶的水平。LPS 处理增加了谷氨酸草酰乙酸转氨酶、谷氨酸丙酮酸转氨酶、乳酸脱氢酶和丙二醛的水平，但 PF 处理阻止了这些增加。LPS 处理还降低了超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的抗氧化水平，但 PF 阻止了这些降低。PF 还通过组织病理学显示可以保护肝脏组织免受损伤。这些结果表明，PF 可以预防 LPS 诱导的肝脏炎症。
