Division of Environmental Science and Ecological Engineering, Korea University, Anam-Dong 5 Ga, 136-713, Seoul, South Korea.
Curr Microbiol. 2011 Jan;62(1):249-54. doi: 10.1007/s00284-010-9698-y. Epub 2010 Jul 7.
The genus Acinetobacter has been recognized to take up exogenous DNA from the environment. In this study, we conducted natural transformation with a novel diesel-degrading Acinetobacter sp. strain, designated strain DR1, using the broad host range plasmid pRK415. Many factors, including temperature, quantities of DNA, and aeration have proven critically important for efficient natural transformation. Interestingly, the Acinetobacter sp. strain DR1 (pRK415) differed both phenotypically and physiologically from the wild-type strain in several regards, including motility, biofilm formation ability, and responses to oxidative stress: the transformed cells were rendered more sensitive to hydrogen peroxide and cumene hydroperoxide, and their motilities and biofilm formation activity were also attenuated. Our data demonstrated that caution should be exercised when conducting genetic manipulation with plasmids, due to the possibility that phenotypic and physiological changes in the host might occur along with the uptake of plasmids.
不动杆菌属已被证实能够从环境中摄取外源 DNA。在这项研究中,我们使用广泛宿主范围的质粒 pRK415 对新型柴油降解不动杆菌菌株 DR1 进行了自然转化。许多因素,包括温度、DNA 量和通气,已被证明对高效自然转化至关重要。有趣的是,不动杆菌属菌株 DR1(pRK415)在表型和生理方面与野生型菌株在多个方面存在差异,包括运动性、生物膜形成能力和对氧化应激的反应:转化细胞对过氧化氢和过氧化枯烯更加敏感,其运动性和生物膜形成活性也减弱。我们的数据表明,在使用质粒进行遗传操作时应谨慎,因为随着质粒的摄取,宿主可能会发生表型和生理变化。