McCarty R, Eisen G, Bartholow C L
Department of Psychology, University of Virginia, Charlottesville 22903-2477.
Physiol Behav. 1991 Mar;49(3):653-6. doi: 10.1016/0031-9384(91)90296-z.
Plasma levels of norepinephrine (NE) and epinephrine (EPI) were measured in adult male Fischer 344 (F-344) laboratory rats exposed to acute motion stress. Two days prior to testing, rats were prepared with chronic tail artery catheters to permit remote sampling of blood from conscious, freely behaving animals. Animals remained in their home cages during the entire testing protocol. After collection of basal blood samples, cages were rotated at 45 revolutions per minute for a 10-minute period each hour for 5 consecutive hours. Additional blood samples were collected immediately after each of the 10-minute rotation stress sessions. Plasma levels of NE remained unchanged from baseline immediately following each of the rotation stress sessions. In contrast, plasma EPI increased significantly above baseline levels following each of the 5 rotation stress sessions. These data indicate that rotation stress provides a selective activation of epinephrine-containing adrenal chromaffin cells as reflected in an increase in plasma EPI but not NE. This stress model could prove valuable in examining the physiological and behavioral consequences of adrenal EPI release in freely behaving animals without the confounding effects of increases in circulating norepinephrine.
在暴露于急性运动应激的成年雄性Fischer 344（F-344）实验大鼠中测量了去甲肾上腺素（NE）和肾上腺素（EPI）的血浆水平。在测试前两天，给大鼠植入慢性尾动脉导管，以便从清醒、自由活动的动物身上远程采集血液样本。在整个测试过程中，动物都待在它们的笼子里。在采集基础血样后，笼子以每分钟45转的速度旋转，每次10分钟，每小时进行一次，连续进行5小时。在每次10分钟的旋转应激试验后立即采集额外的血样。在每次旋转应激试验后，NE的血浆水平与基线相比立即保持不变。相比之下，在5次旋转应激试验中的每一次之后，血浆EPI均显著高于基线水平。这些数据表明，旋转应激可选择性激活含肾上腺素的肾上腺嗜铬细胞，这表现为血浆EPI增加而NE未增加。这种应激模型在研究自由活动动物中肾上腺EPI释放的生理和行为后果时可能具有重要价值，且不会受到循环去甲肾上腺素增加的混杂影响。
