Tc-Mercaptoacetyl-Glu-Glu-Glu-Affibody Z

Epidermal growth factor (EGF) is a cytokine that comprises 53 amino acids (6.2 kDa) and is secreted by ectodermic cells, monocytes, kidneys, and duodenal glands (1). EGF stimulates growth of epidermal and epithelial cells. EGF and at least seven other growth factors and their transmembrane receptor kinases play important roles in cell proliferation, survival, adhesion, migration, and differentiation. The EGF receptor (EGFR) family consists of four transmembrane receptors: EGFR (HER1/erbB-1), HER2 (erbB-2/neu), HER3 (erbB-3), and HER4 (erbB-4) (2). HER1, HER3, and HER4 comprise three major functional domains: an extracellular ligand-binding domain, a hydrophobic transmembrane domain, and a cytoplasmic tyrosine kinase domain. No ligand has been clearly identified for HER2. However, HER2 can be activated as a result of ligand binding to other HER receptors with the formation of receptor homodimers and/or heterodimers (3). HER1 and HER2 are overexpressed on many solid tumor cells such as breast, non-small cell lung, head and neck, and colon cancers (4-6). The high levels of HER1 and HER2 expression on cancer cells are associated with a poor prognosis (7-10). Trastuzumab is a humanized IgG monoclonal antibody (mAb) against the extracellular domain of recombinant HER2 with an affinity constant () of 0.1 nM (11). Trastuzumab is approved for clinical use for anti-cancer therapies in both Europe and North America. In-Trastuzumab, Cy5.5-trastuzumab, and Ga-trastuzumab-F(ab') eveloped for imaging human breast cancer (12-16). However, the pharmacokinetics of the intact radiolabeled mAb, with high liver uptake and slow blood elimination, are generally not ideal for imaging. Smaller antibody fragments, such as Fab or F(ab´), have better imaging pharmacokinetics because they are rapidly excreted by the kidneys. A novel class of recombinant affinity ligands (Affibody molecules) for HER2 was constructed on the basis of the Z-domain residues (58 amino acids) from one of the IgG-binding domains of staphylococcal protein A (17). Affibody molecules exhibit high binding affinity () to HER2 with values <100 pM. Various radiolabeled Affibody molecules have been studied in terms of their ability to image HER2 in tumors [PubMed]. Mercaptoacetyl-Gly-Gly-Gly (MAG3) was used a chelating linker for coupling Tc to Z Affibody (18). Tc-MAG3-Z has been evaluated in nude mice bearing human colon adenocarcinoma tumors, resulting in high tumor/blood and tumor/muscle ratios. However, Tc-MAG3-Z exhibited a high hepatobiliary clearance, which resulted in a high radioactivity in the intestines at 4 h after injection (19). Therefore, glutamic acid was used as the chelating linker to decrease the lipophilicity of Z Affibody to suppress hepatobiliary clearance (20). Tc-Mercaptoacetyl-Glu-Glu-Glu-Affibody Z (Tc-MaEEE-Z) was found to a have favorable biodistribution in nude mice bearing SKOV-3 tumors, resulting in a 3-fold lower hepatobiliary clearance than Tc-MAG3-Z.