汞、甲基汞和铝对大鼠小脑星形胶质细胞培养物中胶质纤维酸性蛋白表达的影响。
Effects of mercury, methylmercury and aluminium on glial fibrillary acidic protein expression in rat cerebellar astrocyte cultures.
作者信息
Toimela T A, Tähti H
机构信息
Department of Biomedical Sciences, University of Tampere, Box 607, FIN-33101 Tampere, Finland.
出版信息
Toxicol In Vitro. 1995 Jun;9(3):317-25. doi: 10.1016/0887-2333(95)00002-p.
The effects of mercuric chloride, methylmercury chloride and aluminium chloride on glial fibrillary acidic protein (GFAP) expression in primary cerebellar astrocyte cultures were studied. GFAP has been found to be a quantitative marker of neuronal injuries on the central nervous system in vivo. The GFAP content of the astrocytes was examined by sandwich ELISA after exposure for 1 wk to various metal concentrations in the culture medium. Cellular injuries were assessed by quantifying the lactate dehydrogenase (LDH) leakage into the culture medium. The expression of GFAP was found at lower dose levels than the leakage of LDH, indicating that the expression of GFAP could be a more sensitive marker of neurotoxicity than LDH leakage. In morphological examination, staining with monoclonal antibody showed GFAP induction after mercury exposure.
研究了氯化汞、甲基氯化汞和氯化铝对原代小脑星形胶质细胞培养物中胶质纤维酸性蛋白（GFAP）表达的影响。GFAP已被发现是体内中枢神经系统神经元损伤的定量标志物。在将星形胶质细胞暴露于培养基中不同金属浓度1周后，通过夹心酶联免疫吸附测定法检测其GFAP含量。通过量化乳酸脱氢酶（LDH）泄漏到培养基中的量来评估细胞损伤。发现GFAP的表达剂量水平低于LDH的泄漏，这表明GFAP的表达可能是比LDH泄漏更敏感的神经毒性标志物。在形态学检查中，用单克隆抗体染色显示汞暴露后GFAP诱导。
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