Wang Z G, Cao Y, D'Urso C, Houghton A N, Ferrone S
Department of Microbiology and Immunology, New York Medical College, Valhalla 10595.
Int J Cancer Suppl. 1991;6:101-5. doi: 10.1002/ijc.2910470720.
Testing of a panel of cultured human melanoma cells with radiolabelled anti-HLA-class-I monoclonal antibodies (MAbs) in a binding assay has shown lack of reactivity of FO-I and SK-MEL-33 cells and low reactivity of SK-MEK-19 cells. SDS-PAGE analysis of the components immunoprecipitated from the 3 intrinsically radiolabelled melanoma cell lines by antibodies to the 2 subunits of HLA-class-I antigens has not detected beta 2-mu in the immunoprecipitates from melanoma cells FO-I and SK-MEL-33 and only a low level of HLA-class-I heavy chain in the immunoprecipitate from SK-MEL-19 cells. Northern blotting analysis with probes specific for HLA-class-I heavy chain and for beta 2-mu indicates that the abnormalities in HLA-class-I-antigen expression reflects a defect at the transcriptional level in FO-I cells and at the post-transcriptional level in SK-MEL-19 and in SK-MEL-33 cells. FO-I, SK-MEL-19 and SK-MEL-33 cells represent useful models to analyze the molecular mechanisms underlying the loss of HLA-class-I-antigen expression which is often associated with malignant transformation of melanocytes and to characterize the role of HLA-class-I antigens in the biology of melanoma cells and in their interactions with effector cells.
在结合试验中，用放射性标记的抗HLA - I类单克隆抗体（MAbs）对一组培养的人黑色素瘤细胞进行检测，结果显示FO - I和SK - MEL - 33细胞无反应性，SK - MEK - 19细胞反应性较低。通过针对HLA - I类抗原的2个亚基的抗体对3种内在放射性标记的黑色素瘤细胞系免疫沉淀的成分进行SDS - PAGE分析，在黑色素瘤细胞FO - I和SK - MEL - 33的免疫沉淀物中未检测到β2 -微球蛋白，在SK - MEL - 19细胞的免疫沉淀物中仅检测到低水平的HLA - I类重链。用针对HLA - I类重链和β2 -微球蛋白的特异性探针进行Northern印迹分析表明，HLA - I类抗原表达异常反映了FO - I细胞在转录水平的缺陷以及SK - MEL - 19和SK - MEL - 33细胞在转录后水平的缺陷。FO - I、SK - MEL - 19和SK - MEL - 33细胞是分析HLA - I类抗原表达缺失背后分子机制的有用模型，HLA - I类抗原表达缺失通常与黑素细胞的恶性转化相关，这些细胞还可用于表征HLA - I类抗原在黑色素瘤细胞生物学及其与效应细胞相互作用中的作用。
