从白头海雕（Haliaeetus leucocephalus）中分离出混合亚型的甲型流感病毒。
Isolation of mixed subtypes of influenza A virus from a bald eagle (Haliaeetus leucocephalus).
机构信息
Department of Veterinary Population Medicine, University of Minnesota, College of Veterinary Medicine, Saint Paul, MN 55108, USA.
出版信息
Virol J. 2010 Jul 28;7:174. doi: 10.1186/1743-422X-7-174.
From April 2007 to March 2008, cloacal swabs were obtained from 246 casualty raptors recovered by various wildlife rehabilitation centers in the United States. The swabs were placed in a virus transport medium and transported to the laboratory on ice packs. At the laboratory, the samples were pooled with each pool consisting of five samples. All pools (n = 50) were screened for the presence of avian influenza virus (AIV) using a real time reverse transcription-polymerase chain reaction (rRT-PCR); one of the pools was found positive. All five samples in this pool were tested individually by rRT-PCR; one sample from a bald eagle was found positive. This sample was inoculated in embryonated chicken eggs for virus isolation and a hemagglutinating virus was isolated. Complete genome sequencing of the isolate revealed a mixed infection with H1N1 and H2N1 subtypes. Further analysis revealed that the PB1-F2 gene sequence of H1N1 virus had the N66S virulence-associated substitution. Further studies on ecology and epidemiology of AIV in raptors are needed to help understand their role in the maintenance and evolution of AIV.
2007 年 4 月至 2008 年 3 月，从美国各地各种野生动物康复中心回收的 246 只受伤猛禽的泄殖腔拭子。将拭子放入病毒运输培养基中，并在冰袋上运送到实验室。在实验室中，将每个包含五个样本的样本池合并，用于使用实时逆转录聚合酶链反应（rRT-PCR）检测禽流感病毒（AIV）的存在；发现一个样本池呈阳性。通过 rRT-PCR 单独测试该池中的所有五个样本；从白头鹰中发现一个样本呈阳性。将该样本接种到鸡胚中进行病毒分离，并分离出血凝病毒。分离株的完整基因组测序显示存在 H1N1 和 H2N1 亚型的混合感染。进一步分析表明，H1N1 病毒的 PB1-F2 基因序列具有 N66S 毒力相关取代。需要进一步研究猛禽中 AIV 的生态学和流行病学，以帮助了解它们在 AIV 的维持和进化中的作用。
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