CNR-IGV, Institute of Plant Genetics, Portici, Italy.
Expert Rev Vaccines. 2010 Aug;9(8):893-911. doi: 10.1586/erv.10.78.
Production of recombinant subunit vaccines from genes incorporated in the plastid genome is advantageous because of the attainable expression level due to high transgene copy number and the absence of gene silencing; biocontainment as a consequence of maternal inheritance of plastids and no transgene presence in the pollen; and expression of multiple transgenes in prokaryotic-like operons. We discuss the core technology of plastid transformation in Chlamydomonas reinhardtii, a unicellular alga, and Nicotiana tabacum (tobacco), a flowering plant species, and demonstrate the utility of the technology for the production of recombinant vaccine antigens.
从整合到质体基因组中的基因生产重组亚单位疫苗具有优势，因为可以通过高转基因拷贝数和不存在基因沉默来达到可实现的表达水平；由于质体的母系遗传和花粉中不存在转基因，因此具有生物封存性；并且可以在原核样操纵子中表达多个转基因。我们讨论了单细胞藻类莱茵衣藻和开花植物烟草的质体转化的核心技术，并证明了该技术在生产重组疫苗抗原方面的实用性。
