Department of Pathology and Immunology, University of Geneva, 1 Rue Michel-Servet, 1211 Geneva 4, Switzerland.
Res Microbiol. 2010 Oct;161(8):706-10. doi: 10.1016/j.resmic.2010.07.003. Epub 2010 Jul 30.
Microcin E492 (MccE492) is an antibacterial protein whose activity on target cells requires ManYZ, the inner membrane component of the mannose permease. We show here that MceA, the polypeptide core of MccE492, stably associates with ManYZ both in the presence and in the absence of MceB, the MccE492 immunity protein. The two known physiological activities of the mannose permease were assayed in cells co-expressing MceA and MceB. Under these conditions, growth on mannose as the sole carbon source is prevented; this was not observed in cells expressing only MceB. In contrast, susceptibility to bacteriophage λ infection was not affected.
微菌素 E492（MccE492）是一种抗菌蛋白，其对靶细胞的活性需要 ManYZ，即甘露糖渗透酶的内膜成分。我们在这里表明，MccE492 的多肽核心 MceA 在有和没有 MccE492 免疫蛋白 MceB 的情况下都能与 ManYZ 稳定结合。甘露糖渗透酶的两种已知生理活性在共表达 MceA 和 MceB 的细胞中进行了检测。在这些条件下，阻止了仅以甘露糖作为唯一碳源的生长；而在仅表达 MceB 的细胞中则没有观察到这种情况。相比之下，噬菌体 λ 感染的敏感性不受影响。
