聚维酮碘（PVPI）对大鼠细菌性腹膜炎的治疗作用。

Araujo Ivana Duval de, Grossi Giovanni Cezar Xavier, Diniz Simone Odília Fernandes, Nunes Tarcizo Afonso, Braga Eduardo Angelo, Cardoso Valbert Nascimento

Medical School, Federal University of Minas Gerais (UFMG), Minas Gerais, Brazil.

Acta Cir Bras. 2010 Aug;25(4):322-7. doi: 10.1590/s0102-86502010000400004.

PURPOSE

To evaluate the effectiveness of the use of the povidone-iodine (PVI) added to the liquid of wash of the peritoneal cavity in the reduction of bacterial absorption and in the remainder non-phagocyted bacteria in the circulating blood of rat.

METHODS

Thirty four Wistar females rats were used, distributed in the following groups: A (n=10), non-treated; B (n=9), wash of the peritoneal cavity with solution of PVI to 1% in saline solution; C (n=15), wash of the cavity with saline solution. After anesthesia, it was made intraperitoneal infusion of solution of Escherichia coli labeled with 99mTc containing 10(8) CFU/ml. After 40 minutes, it was made the treatment, in the group A, manipulation of the viscera; in the group B, irrigation of the peritoneal cavity with warm solution of 1% PVPI to 37,5 degrees C, and in the group C irrigation with warm saline (37,5 degrees C). After 15 minutes of the treatment, blood samples and fragments of liver, spleen and lung was obtained for count of the radioactivity, and animals killed by abdominal aorta section. There were determined the bacterial absorption index and the remainder index in the bloodstream.

RESULTS

Of the total of bacteria infused in the peritoneum, there was absorption of 0,92% (0,14% to 2,13%) in the animals of the group A (controls), 0,49% (0,18% to 0,71%) after use of topical PVPI (group B) and 0,80% (0,04% to 3,8%) after wash with saline solution (group C). There was significant reduction of the absorption when compared the treated animals with PVPI and the controls (p=0,003). Of the total of bacteria absorbed for the circulatory current, the percentile amount of bacteria non-phagocyted in the outlying blood was of 2,9% (1,1% to 17,7%) in the control group, 15,2% (8,3% to 21,4%) in those treated with PVPI (group B) and 6,9% (0,8% to 29,7%) after wash with saline solution (group C), with difference among controls and treated with PVPI (p=0,01).

CONCLUSION

The wash of the cavity peritoneal of mice with solution containing PVPI showed to be capable to reduce the absorption of bacteria by peritoneum of rat; however it seems to interfere with the function of the phagocytic cells for the observation of the increase of viable bacteria in the outlying blood of those animals.

目的

评估在大鼠腹腔冲洗液中添加聚维酮碘（PVI）对减少细菌吸收以及循环血液中剩余未被吞噬细菌的效果。

方法

使用34只雌性Wistar大鼠，分为以下几组：A组（n = 10），未处理；B组（n = 9），用含1% PVI的盐溶液进行腹腔冲洗；C组（n = 15），用盐溶液进行腹腔冲洗。麻醉后，腹腔内注入含10⁸CFU/ml 99mTc标记的大肠杆菌溶液。40分钟后进行处理，A组进行脏器操作；B组用37.5℃的1% PVPI温溶液冲洗腹腔；C组用37.5℃的温盐溶液冲洗。处理15分钟后，采集血液样本以及肝、脾和肺组织碎片用于放射性计数，然后通过切断腹主动脉处死动物。测定细菌吸收指数和血液中的剩余指数。

结果

在注入腹腔的细菌总数中，A组（对照组）动物的细菌吸收率为0.92%（0.14%至2.13%），使用局部PVPI后（B组）为0.49%（0.18%至0.71%），用盐溶液冲洗后（C组）为0.80%（0.04%至3.8%）。与用PVPI处理的动物和对照组相比，细菌吸收有显著降低（p = 0.003）。在循环血液中吸收的细菌总数中，对照组外周血中未被吞噬的细菌百分比为2.9%（1.1%至17.7%），用PVPI处理的动物（B组）为15.2%（8.3%至21.4%），用盐溶液冲洗后（C组）为6.9%（0.8%至29.7%），对照组与用PVPI处理的动物之间存在差异（p = 0.01）。