Rojas L, Zuazaga C
Institute of Neurobiology, University of Puerto Rico, San Juan 00901.
Neurosci Lett. 1991 Apr 1;124(2):195-9. doi: 10.1016/0304-3940(91)90092-8.
Spontaneous openings of acetylcholine receptor channels were investigated using Xenopus myotomal muscle cells in culture and patch clamp recording methods. Two classes of channels with conductances close to 40 and 60 pS and reversal potentials near 0 mV were found to open in the absence of agonist. The open time of the low-conductance channel was about 3 times longer than that of the high-conductance channel. Open times were shorter by a factor of 3-5 than those of acetylcholine-activated channels in Xenopus myocytes and showed no significant voltage dependence. These studies indicate that the two predominant classes of acetylcholine receptor channels in this preparation can open spontaneously.
利用培养的非洲爪蟾肌节肌细胞和膜片钳记录方法，对乙酰胆碱受体通道的自发开放进行了研究。发现在没有激动剂的情况下，有两类电导接近40和60 pS且反转电位接近0 mV的通道会开放。低电导通道的开放时间比高电导通道长约3倍。与非洲爪蟾肌细胞中乙酰胆碱激活的通道相比，开放时间短3至5倍，且无明显电压依赖性。这些研究表明，该制剂中两类主要的乙酰胆碱受体通道能够自发开放。
