用于悬浮双层离子通道记录的石英纳米孔膜。

Quartz nanopore membranes for suspended bilayer ion channel recordings.

机构信息

Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, Utah 84112, USA.

出版信息

Anal Chem. 2010 Sep 1;82(17):7259-66. doi: 10.1021/ac101183j.

PMID:20684522

Abstract

A method is described for fabricating 25-75 mum thick fused quartz membranes containing a single conical shaped nanopore (orifice radius ranging from 10 to 1000 nm). The quartz nanopore membrane (QNM) provides an excellent solid support structure for lipid bilayers in ion channel recordings due to the large electrical resistivity of fused quartz. Electrical measurements demonstrate that the leakage current through 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) bilayers suspended across a 500-1000 nm radius QNM orifice is immeasurably small, corresponding to a bilayer resistance greater than 10(12) ohms. Translocation of single-stranded DNA oligomers (poly dA 50-mer and poly dA 20-mer) through a protein ion channel (alpha-hemolysin) reconstituted in a DPhPC bilayer suspended across the QNM orifice is demonstrated.

摘要

描述了一种制造含有单个圆锥形纳米孔（孔径范围为 10 至 1000nm）的 25-75μm 厚熔融石英膜的方法。由于熔融石英的高电阻率，石英纳米孔膜（QNM）为离子通道记录中的脂质双层提供了极好的固体支撑结构。电测量表明，通过悬浮在 500-1000nm 半径 QNM 孔口的 1,2-二植烷酰基-sn-甘油-3-磷酸胆碱（DPhPC）双层的泄漏电流可忽略不计，对应于大于 10（12）欧姆的双层电阻。通过在悬浮于 QNM 孔口的 DPhPC 双层中再构成的蛋白质离子通道（α-溶血素）穿过单链 DNA 寡聚物（多 dA50 聚体和多 dA20 聚体）进行转运。