Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Org Lett. 2010 Sep 3;12(17):3776-9. doi: 10.1021/ol101408f.
Nonsense codon suppression for unnatural amino acid incorporation requires the preparation of a suppressor aminoacyl-tRNA. Chemical acylation strategies are general but inefficient and arduous. A recent report (J. Am. Chem. Soc. 2007, 129, 15848) showed acylation of RNA mediated by lanthanum(III) using amino acid phosphate esters. The successful implementation of this methodology to full-length suppressor tRNA is described, and it is shown that the derived aminoacyl-tRNA is translationally competent in Xenopus oocytes.
无义密码子抑制用于非天然氨基酸掺入需要制备抑制氨酰-tRNA。化学酰化策略是通用的,但效率低且费力。最近的一份报告(J. Am. Chem. Soc. 2007, 129, 15848)表明,使用氨基酸磷酸酯通过镧(III)介导的 RNA 酰化。描述了该方法在全长抑制 tRNA 中的成功实施,并表明衍生的氨酰-tRNA在非洲爪蟾卵母细胞中具有翻译能力。