仙花饮对金黄地鼠颊囊癌前病变逆转作用的研究。

Role of the Chinese herbal medicine xianhuayin on the reversal of premalignant mucosal lesions in the golden hamster buccal pouch.

机构信息

Department of Stomatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.

出版信息

Int J Oral Sci. 2010 Mar;2(1):53-8. doi: 10.4248/IJOS10013.

DOI:10.4248/IJOS10013

PMID:20690419

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3475595/

Abstract

AIM

To investigate the role of the Chinese herbal medicine Xianhuayin on the reversal of 7,12-dimethylbenz[a]anthracene (DMBA)-induced premalignant mucosal lesions in the oral buccal pouch of golden hamsters.

METHODOLOGY

The animals were randomly divided into a non-diseased control group (n=5) and an experimental group including 50 animals in which the buccal mucosa had been painted with DMBA (0.5% in acetone) to generate an oral mucosa premalignant lesion. Animals in the experimental group were further divided into Xianhuayin-treated group (n=30), untreated premalignant lesion group (n=10) and normal saline (NS)-treated group (n=10). The cheek (buccal) pouch mucosa of the golden hamsters in each group was observed with light and electron microscopy eight weeks after intragastric administration with NS or Xianhuayin.

RESULTS

In the non-diseased control group, the buccal mucosa was keratinized and stratified squamous epithelium under a light microscope. In the untreated premalignant lesion group, variable degrees of epithelial dysplasia was observed. The irregular epithelial mucosa gradually became distinct in the Xianhuayin-treated group. Scanning electronic microscopic (SEM) analysis showed that surface of the cells exhibited honeycomb structures in the hamster of untreated-group. The cells were morphologically irregular, overlapped and loosened in the untreated premalignant lesion group. Most of the cell surface exhibited honeycomb structure in the Xianhuayin-treated group. Transmission electronic microscopic (TEM) analysis showed that buccal mucosal epithelial cells were morphologically regular in the non-diseased control group. Desmosomes and tonofibrils were reduced and the nucleus was morphologically irregular in the untreated premalignant lesion group. In the Xianhuayin-treated group, the widening intercellular gap was gradually reduced, desmosomes and the cells becoming morphologically regular. No significant difference was observed between the hamsters in NS-treated group and those in the untreated premalignant lesion group. Significant therapeutic efficacy was observed in the group receiving Xianhuayin.

CONCLUSION

Xianhuayin is effective in the reversal of DMBA-induced premalignant lesions in the buccal pouch of golden hamsters.

摘要

目的

研究中药仙花饮对 7,12-二甲基苯并[a]蒽（DMBA）诱导的金黄地鼠口腔颊囊黏膜癌前病变逆转的作用。

方法

动物随机分为非疾病对照组（n=5）和实验组（n=50），实验组颊黏膜涂抹 DMBA（丙酮中 0.5%）诱导口腔黏膜癌前病变。实验组动物进一步分为仙花饮治疗组（n=30）、未治疗癌前病变组（n=10）和生理盐水（NS）治疗组（n=10）。每组动物在灌胃 NS 或仙花饮 8 周后，用光镜和电镜观察颊（颊）囊黏膜。

结果

在非疾病对照组中，光镜下颊黏膜为角化复层扁平上皮。在未治疗的癌前病变组中，观察到不同程度的上皮异型增生。在仙花饮治疗组中，不规则的上皮黏膜逐渐变得明显。扫描电子显微镜（SEM）分析显示，未治疗组的细胞表面呈蜂窝状结构。细胞形态不规则，重叠疏松。仙花饮治疗组的大部分细胞表面呈蜂窝状结构。透射电子显微镜（TEM）分析显示，非疾病对照组颊黏膜上皮细胞形态规则。未治疗的癌前病变组桥粒和张力原纤维减少，细胞核形态不规则。在仙花饮治疗组中，细胞间间隙逐渐变窄，桥粒和细胞形态逐渐规则。NS 治疗组和未治疗的癌前病变组之间的差异无统计学意义。仙花饮组疗效显著。