一种用于 Western blot 实验的经济、单侧面抗体孵育方法。

An economical single-sided antibody incubation method for Western blotting.

机构信息

College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.

出版信息

J Virol Methods. 2010 Nov;169(2):409-11. doi: 10.1016/j.jviromet.2010.07.026. Epub 2010 Aug 4.

DOI:10.1016/j.jviromet.2010.07.026

PMID:20691216

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8330846/

Abstract

A simple, single-sided antibody method for incubating primary and secondary antibodies in Western blotting was developed, which generates significant savings on the use of antibodies. Compared with the conventional immersion technique for antibody incubation, the present economical single-sided antibody incubation method resulted in a saving of 80% of antibody use. Besides, the present incubation method did not compromise the Western blot results and was not affected by the expression levels of target proteins.

摘要

本研究开发了一种简单的、单侧面抗体孵育方法，用于 Western blot 中的一抗和二抗孵育，这大大节省了抗体的使用。与传统的抗体孵育浸泡技术相比，本经济的单侧面抗体孵育方法节省了 80%的抗体用量。此外，本孵育方法不影响 Western blot 结果，也不受靶蛋白表达水平的影响。