宫颈上皮内瘤变：端粒酶活性和 hTERT mRNA 的剪接模式。

Cervical intraepithelial neoplasia: Telomerase activity and splice pattern of hTERT mRNA.

机构信息

Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Russia.

出版信息

Biochimie. 2010 Dec;92(12):1827-31. doi: 10.1016/j.biochi.2010.07.015. Epub 2010 Aug 5.

DOI:10.1016/j.biochi.2010.07.015

PMID:20691751

Abstract

Cervical cancers are characterized by the persistence of human papilloma virus (HPV) genome that is found in tissue samples starting from the early stages of tumor progression. Just like in other tumors, the activation of telomerase was observed in cervical carcinomas, but information about its expression was controversial. The aim of this study is to find possible correlations between the presence of HPV sequences, activity of telomerase and expression of different spliced forms of hTERT RNA in cervical intraepithelial neoplasias (CIN). The results show that HPV DNA is present in 60% of normal tissue adjacent to CIN lesions and up to 84% in CIN samples. Telomerase activity was found in 28% of adjacent normal tissue and in 68% of CIN II-III. hTERT RNA that encodes an active enzyme was present almost in all CIN samples. Variations in levels of telomerase activity are possibly not regulated by the splicing forms of hTERT mRNA with deletions.

摘要

宫颈癌的特征是在肿瘤进展的早期阶段，组织样本中就存在人乳头瘤病毒（HPV）基因组的持续存在。就像在其他肿瘤中一样，在宫颈癌中观察到端粒酶的激活，但关于其表达的信息存在争议。本研究旨在寻找 HPV 序列的存在、端粒酶的活性以及不同剪接形式的 hTERT RNA 在宫颈上皮内瘤变（CIN）中的表达之间的可能相关性。结果表明，HPV DNA 存在于 CIN 病变相邻的正常组织的 60%，在 CIN 样本中高达 84%。在 28%的相邻正常组织和 68%的 CIN II-III 中发现了端粒酶活性。编码活性酶的 hTERT RNA 几乎存在于所有 CIN 样本中。端粒酶活性水平的变化可能不受具有缺失的 hTERT mRNA 剪接形式的调节。