转录因子的亚核定位与核内运输
Subnuclear localization and intranuclear trafficking of transcription factors.
作者信息
Zaidi Sayyed K, Medina Ricardo F, Pockwinse Shirwin M, Bakshi Rachit, Kota Krishna P, Ali Syed A, Young Daniel W, Nickerson Jeffrey A, Javed Amjad, Montecino Martin, van Wijnen Andre J, Lian Jane B, Stein Janet L, Stein Gary S
机构信息
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, USA.
出版信息
Methods Mol Biol. 2010;647:77-93. doi: 10.1007/978-1-60761-738-9_4.
Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.
核微环境是细胞核内结构有序的亚核位点，基因表达、复制和修复的调控机制存在于此。这种区室化对于实现调控复合物组合控制的组织和组装所需的化学计量比是必要的。需要综合并系统地应用分子、细胞、生化和体内遗传学方法，才能全面理解生物控制的复杂性。在这里，我们提供了通过原位免疫荧光显微镜来表征调控机制的核组织的方法。
Zotero 插件