State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People's Republic of China.
Clin Oral Investig. 2011 Dec;15(6):983-91. doi: 10.1007/s00784-010-0454-2. Epub 2010 Aug 10.
The purpose of the present study was to evaluate histologically in beagle dogs the healing in acute dehiscence type defects following treatment with open flap debridement (OFD) with or without porous biphasic calcium phosphate (PBCP). Alveolar bone dehiscence defects were surgically created bilaterally at the labial aspects of maxillary third incisors in 12 beagle dogs. After root conditioning with ethylenediaminetetraacetate, PBCP was filled in the defects and the contralaterals were cured with OFD. Two fluorochrome labelings were administered at the 7th and 11th weeks, respectively. Four dogs were killed at the 12, 16, and 24 weeks, respectively. Histological observations were processed through microcomputed tomographic imaging, fluorescence microscope, and light microscopy. The formation of new regenerated tissues was assessed histomorphometrically. The results revealed the healing after treatments with PBCP evidenced a new attachment apparatus and that with OFD supported periodontal repair. In PBCP groups, the amount of new bone varied from 1.15 to 3.86 mm (23-77.2% of the original defect size), while only 0.3 to 1.04 mm (6-20.8%) in OFD group. The amount of new cementum in PBCP varied from 1.18 to 4.16 mm (23.6-82.3%), while only 0.67 to 1.15 mm (13.4-23%) in OFD group. The amount of periodontal ligament in PBCP varied from 1.03 to 4.12 mm (20.6-82.4%), while only 0 to 0.93 mm (0-18.6%) in OFD group. There was significantly more regenerated tissue in PBCP groups compared to OFD procedures (p < 0.01). The present results indicate that PBCP may enhance periodontal regeneration in acute-type labial dehiscence defects.
本研究旨在通过组织学评估犬急性裂开型缺损经开放式清创术（OFD）治疗后有无多孔双相磷酸钙（PBCP）的愈合情况。12 只比格犬双侧上颌第三切牙唇侧牙槽骨骨开窗缺损。经乙二胺四乙酸根管预处理后，将 PBCP 填入缺损，对侧行 OFD 治疗。分别于第 7 周和第 11 周进行两次荧光标记。分别于第 12、16 和 24 周处死 4 只狗。通过 microCT 成像、荧光显微镜和光镜观察进行组织学观察。通过组织形态计量学评估新再生组织的形成。结果显示，PBCP 治疗后形成了新的附着装置，OFD 支持牙周修复。PBCP 组新骨量为 1.15-3.86mm（原缺损大小的 23-77.2%），而 OFD 组仅为 0.3-1.04mm（6-20.8%）。PBCP 组新生牙骨质量为 1.18-4.16mm（23.6-82.3%），而 OFD 组仅为 0.67-1.15mm（13.4-23%）。PBCP 组牙周韧带量为 1.03-4.12mm（20.6-82.4%），而 OFD 组仅为 0-0.93mm（0-18.6%）。PBCP 组再生组织明显多于 OFD 组（p<0.01）。本研究结果表明，PBCP 可能增强急性唇侧裂开型缺损的牙周再生。
