Chelidonis George, Kavantzas Nikolaos, Patsouris Efstratios, Pagaki Eumorfia, Athanasiadou Anna-Maria, Agrogiannis George, Athanasiadou Pauline
1st Pathology Department and Cytology Unit, Medical School, Athens University, Athens, Greece.
Anal Quant Cytol Histol. 2009 Oct;31(5):332-9.
To evaluate, in imprints of resected non-small cell lung cancers (NSCLCs), the relationship between DNA ploidy and E-cadherin/beta-catenin expression with classical prognostic factors.
A total of 45 patients (28 squamous cell carcinomas and 17 adenocarcinomas) who underwent surgical treatment for NSCLC were examined with immunocytochemistry for the expression of E-cadherin/ beta-catenin imprint smears, which also were stained using the Thionin Feulgen procedure in order to evaluate DNA ploidy in the same cases.
Thirty-two (71.1%) of the tumors were classified as aneuploid. E-cadherin-positive expression was observed in 18 (40%) and p-catenin in 21 (46.7%) of the tumors. We found a significant relationship between DNA ploidy and grade (p= 0.005). E-cadherin and beta-catenin expression correlated with grade (p < 0.0001 for both) and also with nodal status (p = 0.004 and p = 0.017, respectively). The mean DNA index was higher for tumors with negative expression of E-cadherin/beta-catenin (p = 0.045 and p = 0.025, respectively).
DNA aneuploidy correlates with poor and moderately differentiated tumors. E-cadherin- and beta-catenin-positive expression is in relation to well-differentiated carcinomas and nodal metastasis. The relationship between DNA ploidy and E-cadherin/beta-catenin needs to be further investigated.
在切除的非小细胞肺癌（NSCLC）印记标本中，评估DNA倍体和E-钙黏蛋白/β-连环蛋白表达与经典预后因素之间的关系。
对45例接受NSCLC手术治疗的患者（28例鳞状细胞癌和17例腺癌）进行免疫细胞化学检查，以检测E-钙黏蛋白/β-连环蛋白印记涂片的表达情况，同时对这些涂片采用硫堇福尔根染色法进行染色，以便评估同一病例中的DNA倍体情况。
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