Lass Christian, Merfort Irmgard, Martin Stefan F
Allergy Research Group, Department of Dermatology, University Medical Center Freiburg, Hauptstrasse, Freiburg, Germany.
Exp Dermatol. 2010 Nov;19(11):1007-13. doi: 10.1111/j.1600-0625.2010.01136.x.
Inflammation is a crucial step in the development of allergic contact dermatitis. The primary contact with chemical allergens, called sensitization, and the secondary contact, called elicitation, result in an inflammatory response in the skin. The ability of contact allergens to induce allergic contact dermatitis correlates to a great extent with their inflammatory potential. Therefore, the analysis of the sensitizing potential of a putative contact allergen should include the examination of its ability and potency to cause an inflammation. In this study, we examined the inflammatory potential of different weak contact allergens and of the strong sensitizer 2,4,6-trinitrochlorobenzene (TNCB) in vitro and in vivo using the contact hypersensitivity model, the mouse model for allergic contact dermatitis. Cytokine induction was analysed by PCR and ELISA to determine mRNA and protein levels, respectively. Inflammation-dependent recruitment of skin-homing effector T cells was measured in correlation with the other methods. We show that the sensitizing potential of a contact allergen correlates with the strength of the inflammatory response. The different methods used gave similar results. Quantitative cytokine profiling may be used to determine the sensitizing potential of chemicals for hazard identification and risk assessment.
炎症是过敏性接触性皮炎发展过程中的关键步骤。与化学过敏原的初次接触（称为致敏）和二次接触（称为激发）会导致皮肤发生炎症反应。接触性过敏原诱发过敏性接触性皮炎的能力在很大程度上与其炎症潜力相关。因此，对一种假定的接触性过敏原致敏潜力的分析应包括检查其引发炎症的能力和效力。在本研究中，我们使用接触性超敏反应模型（过敏性接触性皮炎的小鼠模型）在体外和体内检测了不同弱接触性过敏原以及强致敏剂2,4,6-三硝基氯苯（TNCB）的炎症潜力。通过PCR和ELISA分别分析细胞因子诱导情况，以确定mRNA和蛋白质水平。与其他方法相关联，测量了炎症依赖性皮肤归巢效应T细胞的募集情况。我们发现接触性过敏原的致敏潜力与炎症反应的强度相关。所使用的不同方法得出了相似的结果。定量细胞因子分析可用于确定化学物质的致敏潜力，以进行危害识别和风险评估。
