沙特阿拉伯耐氨苄西林流感嗜血杆菌分离株的外膜蛋白分析。

Outer membrane protein analysis of ampicillin-resistant isolates of Haemophilus influenzae from Saudi Arabia.

机构信息

Department of Forensic and Biomedical Sciences, University of Lincoln, Lincoln, United Kingdom.

出版信息

J Infect Public Health. 2008;1(1):45-50. doi: 10.1016/j.jiph.2008.08.002. Epub 2008 Oct 27.

DOI:10.1016/j.jiph.2008.08.002

PMID:20701845

Abstract

Haemophilus influenzae isolates characterized in a previously published study from the Kingdom of Saudi Arabia were analysed by outer membrane protein (OMP) profiling. Isolates from patients with confirmed respiratory tract infections were investigated. Antibiotic susceptibility tests in vitro showed 25/129 (19.4%) had various degrees of reduced susceptibility to ampicillin although all were fully susceptible to ceftazidime and ciprofloxacin. OMP profiles of the beta-lactamase mediated ampicillin-resistant and beta-lactamase negative; ampicillin intermediate resistant strains (BLNAI) isolated were investigated. Dendrograms of scanned SDS-PAGE of these strains showed 15 different groupings from the 15 non-typable (NTHi) isolates tested demonstrating a high degree of heterogeneity whereas the 5 Hib isolates demonstrated significantly closer relatedness and were probably clonal. The present study demonstrates the groupings of H. influenzae strains by OMP profile analysis which did not correlate with the beta-lactamase production ability, BLNAI isolates, geographical origin or biotype.

摘要

在之前发表的一项沙特阿拉伯王国的研究中，对流感嗜血杆菌分离株进行了外膜蛋白（OMP）分析。对确诊为呼吸道感染的患者的分离株进行了研究。体外抗生素药敏试验显示，129 株中有 25 株（19.4%）对氨苄西林的敏感性有不同程度降低，尽管所有菌株对头孢他啶和环丙沙星均完全敏感。对产β-内酰胺酶介导的氨苄西林耐药和β-内酰胺酶阴性；氨苄西林中介耐药菌株（BLNAI）进行了 OMP 谱分析。对这些菌株进行 SDS-PAGE 扫描的聚类分析显示，在 15 株非定型（NTHi）分离株中有 15 种不同的分组，显示出高度的异质性，而 5 株 Hib 分离株显示出明显的亲缘关系，可能是克隆的。本研究通过 OMP 图谱分析显示，流感嗜血杆菌菌株的分组与β-内酰胺酶产生能力、BLNAI 分离株、地理位置或生物型无关。