地衣芽孢杆菌新型耐热锰过氧化物歧化酶。
A novel, thermostable manganese-containing superoxide dismutase from Bacillus licheniformis.
机构信息
Department of Extremophilic Bacteria, Institute of Microbiology, Bulgarian Academy of Sciences, 26, Acad. G. Bonchev Str., 1113, Sofia, Bulgaria.
出版信息
Biotechnol Lett. 2010 Dec;32(12):1893-6. doi: 10.1007/s10529-010-0368-8. Epub 2010 Aug 12.
A new, thermostable superoxide dismutase (SOD) from Bacillus licheniformis M20, isolated from Bulgarian mineral springs, was purified 11-fold with 11% recovery of activity. From native PAGE and SDS-PAGE, the enzyme was composed of two subunits of 21.5 kDa each. The SOD was inhibited only by NaN(3), which suggested that this SOD is of the manganese superoxide dismutase type. The purified enzyme had maximum activity at pH 8 and 55°C. The half-life of the SOD was 10 min at 95°C.
从保加利亚矿泉中分离出的地衣芽孢杆菌 M20 中存在一种新型热稳定超氧化物歧化酶（SOD），经过 11 倍纯化和 11%的活性回收率，该酶的活性得到了显著提高。经 native PAGE 和 SDS-PAGE 分析，该酶由两个 21.5 kDa 的亚基组成。该 SOD 仅被NaN(3)抑制，这表明它属于锰超氧化物歧化酶类型。纯化后的 SOD 在 pH 值为 8 和 55°C 时具有最大的活性。该 SOD 在 95°C 下的半衰期为 10 分钟。
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