Ohajuruka O A, Palmquist D L
Dept. Dairy Sci., Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
J Anim Sci. 1991 Apr;69(4):1726-32. doi: 10.2527/1991.6941726x.
Recovery of hentriacontane (C31 alkane) in feces as influenced by amount consumed and level of dietary fat was examined in a 2 x 2 factorial study in a 4 x 4 Latin square. Treatments were 1) alfalfa hay:concentrate (70:30; AH); 2) AH + calcium soap (500 g/d); 3) grass hay:concentrate (70:30, GH); and 4) GH + calcium soap. Fat did not influence C31 recovery in feces. Fecal recovery (grams/day) decreased quadratically with increasing C31 intake. Compared with AIA, which was 100% recovered in feces, DM digestibility was estimated more accurately (P less than .05) by AIA than by C31. In a second experiment, site of loss of alkane in the intestinal tract was examined by dosing C32 into the rumen or duodenum. Recovery was lower with ruminal dosing, suggesting ruminal loss of the marker. Alkanes are potentially useful markers of particulate matter in the digestive tract; however, documentation of their behavior in a wider range of diets is needed.
在一项4×4拉丁方设计的2×2析因研究中，考察了食用量和日粮脂肪水平对粪便中三十一烷（C31烷烃）回收率的影响。处理方式为：1）苜蓿干草：精料（70:30；AH）；2）AH + 钙皂（500克/天）；3）禾本科干草：精料（70:30，GH）；4）GH + 钙皂。脂肪对粪便中C31的回收率没有影响。随着C31摄入量的增加，粪便回收率（克/天）呈二次方下降。与在粪便中100%回收的AIA相比，用AIA估算干物质消化率比用C31更准确（P < 0.05）。在第二个实验中，通过向瘤胃或十二指肠灌注C32来考察烷烃在肠道内的损失部位。瘤胃灌注时回收率较低，表明标记物在瘤胃中损失。烷烃可能是消化道中颗粒物的有用标记物；然而，需要记录它们在更广泛日粮中的行为。
