土耳其东部牛皮蝇蛆病的血清学和分子调查。

A serological and molecular survey of cattle hypodermosis in east-Turkey.

机构信息

Department of Parasitology, Faculty of Veterinary Medicine, University of Ataturk, Erzurum, Turkey.

出版信息

Vet Parasitol. 2010 Oct 29;173(3-4):287-91. doi: 10.1016/j.vetpar.2010.07.009. Epub 2010 Jul 30.

DOI:10.1016/j.vetpar.2010.07.009

PMID:20724076

Abstract

The aim of this study was to determine the seroprevalence of hypodermosis in cattle in East-Turkey and to identify Hypoderma species using morphological and molecular methods. For this purpose, a total of 778 serum samples of cattle were collected from Erzurum provinces of east-Turkey from December 2008 to February 2009. The sera were analyzed using a Hypodermin C antigen by means of indirect ELISA. In addition, 10 Hypoderma spp. larvae were collected from slaughtered animals in the abattoir for morphological identification and molecular characterization on the basis of mitochondrial CO1 gene sequence analysis and PCR-RFLP. Two hundred and twenty three (28.6%) out of 778 cattle were seropositive for hypoderma antibodies. All positive cattle were female of a local breed. Seven out of 10 Hypoderma larvae were morphologically classified as third instar larvae (L3) of H. bovis and 3 were classified as L3 of H. lineatum. The TaqI restriction enzyme was used to differentiate the Hypoderma species on the basis of the 438 and 250 bp bands for H. bovis and the 488 and 200 bp bands for H. lineatum resulting from PCR-RFLP. According to the alignment of the mitochondrial CO1 sequences of the Hypoderma species and the PCR-RFLP results, all examined larva samples were classified as H. bovis.

摘要

本研究旨在确定土耳其东部地区牛的皮下蝇蛆病血清流行率，并使用形态学和分子方法鉴定 Hypoderma 物种。为此，于 2008 年 12 月至 2009 年 2 月从土耳其东部埃尔祖鲁姆省采集了 778 份牛血清样本。使用间接 ELISA 用 Hypodermin C 抗原分析血清。此外，从屠宰场屠宰的动物中收集了 10 头 Hypoderma spp. 幼虫，用于形态鉴定和分子特征分析，基于线粒体 CO1 基因序列分析和 PCR-RFLP。在 778 头牛中，有 223 头（28.6%）对皮下蝇蛆抗体呈阳性。所有阳性牛均为当地品种的雌性。10 头 Hypoderma 幼虫中有 7 头形态学上被归类为第三期幼虫（L3）的 H. bovis，3 头归类为 H. lineatum 的 L3。根据 TaqI 限制酶在 H. bovis 的 438 和 250 bp 带以及 H. lineatum 的 488 和 200 bp 带的 PCR-RFLP 结果，可区分 Hypoderma 物种。根据 Hypoderma 物种的线粒体 CO1 序列比对和 PCR-RFLP 结果，所有检查的幼虫样本均被归类为 H. bovis。