Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Sep 15;878(26):2375-8. doi: 10.1016/j.jchromb.2010.07.005. Epub 2010 Jul 18.
A porous polymethacrylate ester-based monolithic column for capillary electrochromatography (CEC) was designed by mean of in situ co-polymerizing lauryl methacrylate (LMA), ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) in a ternary porogenic solvent including cyclohexanol, 1,4-butanediol and water. After investigating the influence factors of the CEC monolithic columns, four flavonoids (i.e., Rutin, Quercetin, Kaempferol, and Quercitrin) were separated and assayed to evaluate this monolithic column with CEC method. Under optimum conditions, the CEC method exhibited high separation efficiency, with rapid separation time of 3-4 min, for the four flavonoid samples using 10mM phosphate buffer containing 70% acetonitrile (pH 9.0). Importantly, the proposed method could provide a promising approach for rapid separation and detection in biomedicine.
一种基于多孔聚甲基丙烯酸酯酯的整体柱用于毛细管电色谱（CEC），通过在包含环己醇、1,4-丁二醇和水的三元致孔溶剂中原位共聚甲基丙烯酸十二酯（LMA）、二乙二醇二甲基丙烯酸酯（EDMA）和 2-丙烯酰胺-2-甲基-1-丙磺酸（AMPS）设计而成。考察了 CEC 整体柱的影响因素后，采用 CEC 法分离和测定了四种黄酮类化合物（芦丁、槲皮素、山奈酚和槲皮苷），以评估该整体柱。在最佳条件下，CEC 法对四种黄酮类化合物样品在 10mM 磷酸盐缓冲液中（pH 9.0）含有 70%乙腈时，分离效率高，分离时间短，仅需 3-4min。重要的是，该方法为生物医学中的快速分离和检测提供了一种很有前途的方法。
