Malicka Joanna, Gryczynski Ignacy, Gryczynski Zygmunt, Lakowicz Joseph R
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 2120.
J Phys Chem B. 2004 Dec 16;108(50):19114-19118. doi: 10.1021/jp047136u.
We studied surface plasmon-coupled emission (SPCE) of 2,5-diphenyl-1,3,4-oxadiazole (PPD) using a 20 nm aluminum film deposited on a quartz substrate. The directional SPCE UV fluorescence occurs within a narrow angle at 57° from the normal to the coupling hemicylindrical prism. This radiation is almost completely p-polarized, consistent with its origin from surface plasmons. These surface plasmons are induced by excited PPD molecules. The coupling of excited fluorophore dipoles with the aluminum is highly efficient, exceeding 50%. Different fluorescence emission wavelengths are emitted at slightly different angles on the prism, providing intrinsic spectral resolution. SPCE fluorescence on thin aluminum films can be used with many UV absorbing and emitting fluorophores.
我们使用沉积在石英衬底上的20纳米铝膜研究了2,5-二苯基-1,3,4-恶二唑（PPD）的表面等离子体耦合发射（SPCE）。定向SPCE紫外荧光在与耦合半圆柱棱镜法线成57°的窄角度内发生。这种辐射几乎完全是p偏振的，这与其源自表面等离子体一致。这些表面等离子体由激发的PPD分子诱导产生。激发的荧光团偶极子与铝的耦合效率很高，超过50%。在棱镜上，不同的荧光发射波长在略微不同的角度发射，提供了固有的光谱分辨率。薄铝膜上的SPCE荧光可与许多紫外吸收和发射荧光团一起使用。
